Graphene-Enhanced 3D Chemical Mapping of Biological Specimens at Near-Atomic Resolution
Graphene-Enhanced 3D Chemical Mapping of Biological Specimens at Near-Atomic Resolution
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DOI：
10.1002/adfm.201801439
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发表时间：
2018-08-08
影响因子：
19
通讯作者：
Fu, Jing
中科院分区：
文献类型：
--
作者：
Adineh, Vahid R.;Zheng, Changxi;Fu, Jing
细胞内单个原子的直接成像对于理解生物体中的基本物理和化学过程具有巨大的潜力。在这里，报道了一种通过引入石墨烯封装方法来伪装低电导率屏障的电绝缘生物细胞成像的新方法。在使用基于水膜的方案成功涂覆后，石墨烯的电性质使得能够进行用于原子探针层析成像（APT）的电压脉冲场蒸发。从Au纳米颗粒和耐药细菌细胞制备的低导电样品已被测试。第一次，还观察到显着的石墨烯增强的APT质量分辨能力，证实了3D数据的改进的组成准确性。2D材料封装的引入为从纳米膜和纳米级生物结构制备样品的突破方向奠定了基础，用于随后的3D近原子表征。
The direct imaging of individual atoms within the cellular context holds great potential for understanding the fundamental physical and chemical processes in organisms. Here, a novel approach for imaging of electrically insulated biological cells by introducing a graphene encapsulation approach to disguise the low-conductivity barrier is reported. Upon successful coating using a water-membrane-based protocol, the electrical properties of the graphene enable voltage pulsing field evaporation for atom probe tomography (APT). Low conductive specimens prepared from both Au nanoparticles and antibiotic-resistant bacterial cells have been tested. For the first time, a significant graphene-enhanced APT mass resolving power is also observed confirming the improved compositional accuracy of the 3D data. The introduction of 2D materials encapsulation lays the foundation for a breakthrough direction in specimen preparation from nanomembrane and nanoscale biological architectures for subsequent 3D near-atomic characterization.