PERMISSIVE ROLE OF INTERLEUKIN-3 (IL-3) IN PROLIFERATION AND DIFFERENTIATION OF MULTIPOTENTIAL HEMATOPOIETIC PROGENITORS IN CULTURE
PERMISSIVE ROLE OF INTERLEUKIN-3 (IL-3) IN PROLIFERATION AND DIFFERENTIATION OF MULTIPOTENTIAL HEMATOPOIETIC PROGENITORS IN CULTURE
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DOI：
10.1002/jcp.1041240203
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发表时间：
1985-01-01
影响因子：
5.6
通讯作者：
IHLE, JN
中科院分区：
文献类型：
--
作者：
SUDA, T;SUDA, J;IHLE, JN
在5-氟尿嘧啶(FU)处理的小鼠脾细胞的甲基纤维素培养体系中，观察了白细胞介素3(IL-3)对造血祖细胞克隆形成的影响。纯化的IL-3支持多种类型的多系集落的生长，包括原始细胞集落。集落类型与商陆-有丝分裂原脾细胞条件培养液(PWM-SCM)相似，但IL-3对嗜酸性粒细胞和中性粒细胞的支持作用更强。细胞接种后7天延迟加入IL-3可使集落数降至0天加入IL-3组的1/2。它不改变晚期出现的多潜能原始细胞集落的增殖和分化特征。IL-3不能刺激造血祖细胞活跃增殖，但对其持续增殖是必需的。IL-3的这种允许作用与干细胞增殖的随机模型是一致的，该模型以随机进入细胞周期为特征。IL-3也支持通过显微操作从原始细胞克隆中分离的单个细胞的多系克隆的生长。IL-3直接作用于多潜能祖细胞。对来自配对祖细胞的集落的分析显示，不同的谱系表达，符合干细胞分化的随机模型。
The effects of interleukin-3 (IL-3) on colony formation by hemopoietic progenitors were studied in methylcellulose cultures of spleen cells from 5-fluorouracil (FU)-treated mice. Purified IL-3 supported the growth of various types of multilineage colonies including blast cell colonies. The types of colonies were similar to those supported by pokeweed-mitogen spleen cell conditioned medium (PWM-SCM), except that IL-3 supported eosinophil and neutrophil expression better. Delayed addition of IL-3 to cultures 7 days after cell plating decreased the number of colonies to 1/2 the number in cultures with IL-3 added on day 0. It did not alter the proliferative and differentiation characteristics of the late emerging multipotential blast cell colonies. IL-3 does not trigger hemopoietic progenitors into active cell proliferation but is necessary for their continued proliferation. This permissive role of IL-3 is consistent with a stochastic model of stem cell proliferation which features random entry into cell cycle. IL-3 also supported the growth of multilineage colonies from single cells isolated from blast cell colonies by micromanipulation. IL-3 acts directly on multipotential progenitors. Analysis of colonies derived from paired progenitors revealed disparate lineage expression and was in accordance with the stochastic model of stem cell differentiation.