Differential cardiac effects of growth hormone and insulin-like growth factor-1 in the rat - A combined in vivo and in vitro evaluation
Differential cardiac effects of growth hormone and insulin-like growth factor-1 in the rat - A combined in vivo and in vitro evaluation
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DOI：
10.1161/01.cir.93.4.800
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发表时间：
1996-02-15
期刊：
影响因子：
37.8
通讯作者：
Douglas, PS
中科院分区：
文献类型：
--
作者：
Cittadini, A;Stromer, H;Douglas, PS
Background Despite their increasing clinical use and recent evidence that growth hormone (GH) and insulin-like growth factor-1 (IGF-1) target the heart, there has been no systematic investigation of the effects of GK and IGF-1 on the cardiovascular system.Methods and Results Sixty normal but growing adult female rats were randomized to receive 4 weeks of treatment with GH (3.5 mg . kg(-1). d(-1)), IGF-1 (3 mg . kg(-1). d(-1)), a combination of the two, or placebo. Transthoracic echocardiograms were performed at baseline and at 2 weeks and 4 weeks of treatment. After the final echocardiography, rats underwent either closed-chest left ventricular (LV) catheterization or Langendorff perfusion studies. Myocyte diameter and interstitial tissue fraction were assessed by morphometric histology. Echocardiographic and ex vivo data demonstrated a LV hypertrophic response in all three groups of treated animals that was most marked in the GH group, which alone exhibited a concentric growth pattern (relative wall thickness, 0.52 versus 0.42 to 0.44 in the other groups; P
