Identification, full-length genome sequencing, and field survey of citrus vein enation virus in Italy
Identification, full-length genome sequencing, and field survey of citrus vein enation virus in Italy
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DOI：
10.36253/phyto-12180
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发表时间：
2021-09-13
影响因子：
2.4
通讯作者：
Navarro, Beatriz
中科院分区：
文献类型：
--
作者：
Minutolo, Maria;Cinque, Maria;Navarro, Beatriz
柑橘静脉病毒(CVEV)在西班牙被发现，随后在亚洲、美洲和澳大利亚的几个柑橘产区也有报道。在这里，首次记录了意大利发生的CVEV。用高通量测序方法测定了CVEV意大利分离株(14Q)的全基因组序列，并通过RT-PCR和嫁接转移到指示植物上证实了病毒的存在，接种6个月后病毒从指示植物中恢复过来。基于不同国家CVEV分离株全长基因组的系统发育分析表明，它们之间的亲缘关系基于地理来源而不是寄主，意大利分离物与西班牙分离物的亲缘关系比与其他分离物的亲缘关系更近。一项田间调查显示，在坎帕尼亚地区(意大利南部)的一些地区存在CVEV，普遍侵染柠檬树。在本次调查的框架内，金橘首次被鉴定为CVEV的宿主。到目前为止，在田间还没有观察到任何症状。无症状宿主的感染和意大利现有的蚜虫物种的传播增加了病毒进一步传播的风险。
Citrus vein enation virus (CVEV) was described in Spain and then it has been reported in several citrus growing areas of Asia, America and Australia. Here, the occurrence of CVEV in Italy has been documented for the first time. The full genome sequence of a CVEV Italian isolate (14Q) was determined by high-throughput sequencing and the presence of the virus was confirmed by RT-PCR and graft-transmission to indicator plants, from which the virus was recovered six-months post-inoculation. Phylogenetic analysis based on the full-length genome of CVEV isolates from different countries showed that they are phylogenetically related to each other based on their geographic origin, rather than on their host and that the Italian isolate is more closely related to the Spanish isolate than to the other ones. A field survey revealed the presence of CVEV in some areas of Campania region (southern Italy), prevalently infecting lemon trees. In the frame of this survey, kumquat was identified for the first time as a host of CVEV. No symptoms were observed in the field so far. The infection of asymptomatic hosts and the transmission by aphid species present in Italy increase the risk that the virus could further spread.