The Effect of Tong-Xie-Yao-Fang on Intestinal Mucosal Mast Cells in Postinfectious Irritable Bowel Syndrome Rats.
The Effect of Tong-Xie-Yao-Fang on Intestinal Mucosal Mast Cells in Postinfectious Irritable Bowel Syndrome Rats.
复制标题
痛泻要方对感染后肠易激综合征大鼠肠粘膜肥大细胞的影响
DOI：
10.1155/2017/9086034
复制
发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Tang X
中科院分区：
文献类型：
--
作者：
Ma X;Wang X;Kang N;Chen T;Ji H;Lv L;Yin X;Tian Y;Zheng R;Duan Y;Wang F;Tang X
Objective.探讨痛泻要方对感染后肠易激综合征（PI-IBS）大鼠肠黏膜肥大细胞的影响。设计采用多刺激法建立PI-IBS大鼠模型。然后分别以2.5、5.0和10.0 g·kg-1·d-1剂量灌胃给药14 d。根据腹部退缩反射（AWR）评分和粪便含水量（FWC）评估肠道敏感性。检测肠黏膜肥大细胞计数、类胰蛋白酶和c-Fos免疫荧光染色，并检测血清IL-1β、TNF-α和组胺水平。结果PI-IBS大鼠的AWR反应性和FWC均显著增加。PI-IBS大鼠肠黏膜肥大细胞活化率明显升高，血清TNF-α和组胺水平明显升高，通心复方可逆转PI-IBS引起的肥大细胞活化及血清TNF-α和组胺水平的变化。同时类胰蛋白酶和c-Fos表达也下调。结论TXYF通过减轻行为痛觉过敏和抗腹泻作用改善PI-IBS症状，其作用机制与其抑制粘膜肥大细胞的活化、下调类胰蛋白酶和c-Fos的表达、降低血清TNF-α和组胺水平有关。
Objective. To investigate the effects of Tong-Xie-Yao-Fang (TXYF) on intestinal mucosal mast cells in rats with postinfectious irritable bowel syndrome (PI-IBS). Design. PI-IBS rat models were established using a multistimulation paradigm. Then, rats were treated with TXYF intragastrically at doses of 2.5, 5.0, and 10.0 g·kg−1·d−1 for 14 days, respectively. Intestinal sensitivity was assessed based on abdominal withdrawal reflex (AWR) scores and fecal water content (FWC). Mast cell counts and the immunofluorescence of tryptase and c-Fos in intestinal mucosa were measured; and serum IL-1β, TNF-α, and histamine levels were determined. Results. AWR reactivity and FWC which were significantly increased could be observed in PI-IBS rats. Remarkably increased mast cell activation ratio in intestinal mucosa, together with increased serum TNF-α and histamine levels, could also be seen in PI-IBS rats; furthermore, PI-IBS-induced changes in mast cell activation and level of serum TNF-α and histamine could be reversed by TXYF treatment. Meanwhile, tryptase and c-Fos expression were also downregulated. Conclusion. TXYF improves PI-IBS symptoms by alleviating behavioral hyperalgesia and antidiarrhea, the underlying mechanism of which involves the inhibitory effects of TXYF on activating mucosal mast cells, downregulating tryptase and c-Fos expression, and reducing serum TNF-α and histamine levels.