Conserved temperature-dependent expression of RNA-binding proteins in cyanobacteria with different temperature optima
Conserved temperature-dependent expression of RNA-binding proteins in cyanobacteria with different temperature optima
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DOI：
10.1016/s0378-1097(03)00503-2
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发表时间：
2003-08-08
影响因子：
2.1
通讯作者：
Sato, N
中科院分区：
文献类型：
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作者：
Ehira, S;Hamano, T;Sato, N
RHP基因编码具有单一RNA识别基序的小RNA结合蛋白，已知在低温下可变鱼腥藻M3的表达增加。RbpA1基因的5‘-非翻译区参与了低温调节。我们比较了三种具有不同温度最适的蓝藻，即一种中温的鱼腥藻，对RBP基因的调控。PCC7120、嗜热长链球菌BP-1和南极嗜冷菌株Oscillatoria sp.苏1。在鱼腥藻7120和长尾鱼腥藻中，所有的RBP基因序列都是已知的，其中一些RBP基因的5‘-非编码区序列与rbpA1的5’-非编码区序列具有很高的相似性。我们发现这些RBP基因的转录本和蛋白产物在低温下积累。此外，在低温条件下，RHP基因在振荡藻中的表达增加。苏1。这表明RBP基因的冷调节机制在不同类群、具有不同最适温度的不同种类的蓝藻中是共同的。(C)2003年，埃尔塞维尔科学公司代表欧洲微生物学会联合会出版。
The expression of the rhp genes, which encode small RNA-binding proteins with a single RNA-recognition motif, is known to increase at low temperature in Anabaena variabilis M3. The 5'-untranslated region (UTR) of the rbpA1 gene is involved in the cold-regulation. We compared the regulation of the rbp genes in three strains of cyanobacteria having different temperature optima, namely, a mesophilic strain Anabaena sp. PCC 7120, a thermophilic strain Thermosynechococcus elongatus BP-1, and a psychrophilic Antarctic strain Oscillatoria sp. SU1. In Anabaena 7120 and T elongatus, all the rbp gene sequences are known, and the 5'-UTR sequences of some rbp genes have a high similarity to the 5'-UTR of rbpA1. We found that transcripts as well as protein products of these rbp genes accumulated at low temperature. In addition, the expression of rhp genes increased at low temperature in the Oscillatoria sp. SU1. This suggests that a mechanism of cold-regulation of rbp genes is common among various species of cyanobacteria that belong to different taxa and have different temperature optima. (C) 2003 Published by Elsevier Science B.V. on behalf of the Federation of European Microbiological Societies.