Patterns of epidermal growth factor receptor amplification in malignant gliomas.
Patterns of epidermal growth factor receptor amplification in malignant gliomas.
复制标题
DOI：
--
复制
发表时间：
1996-04
期刊：
影响因子：
--
通讯作者：
G. Sauter;T. Maeda;F. Waldman;R. Davis;B. Feuerstein
中科院分区：
文献类型：
--
作者：
G. Sauter;T. Maeda;F. Waldman;R. Davis;B. Feuerstein
表皮生长因子受体（EGFR）基因扩增是恶性胶质瘤的常见现象。我们发现，18 29级3和4级胶质瘤EGFR扩增时，采用荧光原位杂交检测。扩增模式表明，在大多数情况下，扩增子包含在双微小染色体内。EGFR拷贝数在单个病例内扩增细胞中可相差20倍。多体性7经常发生在EGFR扩增和未扩增的细胞中。超过三分之一的病例有<或= 10%的EGFR扩增细胞，并且这些病例很可能无法通过不逐个细胞检查DNA的方法进行鉴定。
Amplification of the gene for epidermal growth factor receptor (EGFR) is a common finding in malignant gliomas. We found that 18 of 29 grade 3 and grade 4 gliomas had EGFR amplification when assayed using fluorescence in situ hybridization. The amplification pattern suggests that the amplicon is contained within double minute chromosomes in most cases. EGFR copy number can differ by 20-fold in amplified cells within a single case. Polysomy 7 occurs frequently in both EGFR-amplified and -unamplified cells. More than one-third of the cases had < or = 10 percent of cells with amplified EGFR, and it is likely that these cases would not have been identified by methods that do not examine DNA on a cell by cell basis.