Effect of substance P in cat dorsal horn neurones activated by noxious stimuli
Effect of substance P in cat dorsal horn neurones activated by noxious stimuli
复制标题
P物质对伤害性刺激激活的猫背角神经元的影响
DOI：
10.1016/0006-8993(77)90245-1
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发表时间：
1977
期刊：
影响因子：
2.9
通讯作者：
V. Miletić
中科院分区：
文献类型：
--
作者：
M. Randić;V. Miletić
P物质，最初从马的大脑和肠道分离的肽yon欧拉和Gaddum 4和最近的结构特点2，被发现在一个较高的浓度在背比在腹roots1，5，13，16。这一事实导致Lembeck 13提出P物质可能是初级传入纤维释放的化学递质。Otsuka及其合作者在详细的电生理和生物化学研究中证明：（1）猫脊髓背角内P物质含量最高，即初级传入纤维的终止部位;（2）切断和/或结扎背根后，背角内P物质含量明显降低;（3）外源性P物质对蛙运动神经元和新生大鼠10、11、15、1s、ag的运动神经元均有去极化作用。此外，有证据表明，合成物质P的微离子电渗应用具有很强的，虽然缓慢，兴奋作用，在猫脊髓和楔状核的背角中测试的单位的大约一半7，12。有趣的是，P物质也使初级传入神经的髓内部分去极化，尽管这一观察结果的功能意义尚不清楚14。最近，H6kfelt等人通过免疫组织化学技术检查了P物质的细胞定位。他们已经证明了P物质阳性纤维的非常致密的网络的发生，特别是在猫腰骶脊髓的Lissauer束和层I-III。此外，他们还发现，在局部应用秋水仙碱或压迫靠近脊神经节的背根后，在一些小的神经元细胞体中观察到P物质阳性荧光。与高桥和大冢的生物测定研究一致，他们发现背根切断术后，Lissauer氏束和I-III层中P物质阳性纤维的数量显着减少，19。P物质阳性纤维在Lissauer氏束和I-III层的生理作用目前尚不清楚。然而，最近有一种说法认为它们可能与疼痛感受有关6。亨利6发现，P物质的微电泳应用于单个单位，位于猫的Rexed的板层IV-VI
Substance P, a peptide originally isolated from the horse brain and gut by yon Euler and Gaddum 4 and recently structurally characterized 2, was found at a higher concentration in the dorsal than in ventral roots1, 5, 13, 16. This fact led Lembeck 13 to suggest that substance P may be the chemical transmitter released from primary afferent fibres. A new support for this concept has been provided by Otsuka and his collaborators who demonstrated in detailed electrophysiological and biochemical studies that:(1) the highest concentration of substance P is present in the dorsal horn of the cat spinal cord, the region where the primary afferent fibres terminate;(2) following ligation and/or sectioning of the dorsal roots the substance P level of the dorsal horn was markedly reduced; and (3) exogenously applied substance P has a depolarizing effect on both frog motoneurones and motoneurones of the newborn rat 10, 11, 15, 1s, ag. In addition, evidence has been presented that microiontophoretic application of synthetic substance P has a strong, although slow, excitatory action in about half of the units tested in the dorsal horn of the cat spinal cord and cuneate nucleus 7, 12. Interestingly, substance P also depolarizes the intramedullary portion of the primary afferents, although the functional significance of this observation is unclear 14.The cellular localization of substance P has recently been examined by immunohistochemical techniques by H6kfelt et al. a, 9. They have demonstrated the occurrence of a very dense network of substance P-positive fibres particularly in Lissauer's fasciculus and laminae l-lII of the cat lumbosacral spinal cord. In addition they have found that after local application of colchicine or compression of the dorsal root close to the spinal ganglion, substance P-positive fluorescence was observed in some of the small neuronal cell bodies. In agreement with the bioassay studies of Takahashi and Otsuka, they found a marked decrease in the number of substance P-positive fibres in Lissauer's tract and laminae I-III after a dorsal root rhizotomy x, 19. The physiological role of substance P-positive fibres in Lissauer's fasciculus and laminae I-III is at persent unknown. However, there is a recent suggestion that they may be related to pain preception 6. Henry 6 has found that microelectrophoretic application of substance P to single units, located in Rexed's laminae IV-VI of the cat