Dual effects of interleukin-1β on N-methyl-D-aspartate-induced retinal neuronal death in rat eyes
Dual effects of interleukin-1β on N-methyl-D-aspartate-induced retinal neuronal death in rat eyes
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DOI：
10.1016/s0006-8993(01)02706-8
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发表时间：
2001-08-10
期刊：
影响因子：
2.9
通讯作者：
Tanihara, H
中科院分区：
文献类型：
--
作者：
Kido, N;Inatani, M;Tanihara, H
In this study we determine if interleukin-1 beta (IL-1 beta) modulates N-methyl-D-aspartate (NMDA)-induced retinal damage. Sprague-Dawley rats were anesthetized with inhalation of halothane, after which a single injection of 5 mul of IL-1 beta (0.1 to 10 ng/eye) (and/or IL-1 receptor antagonist (IL-1ra)) for experimental eyes was administered. Two days later (or simultaneously), NMDA (20 nmol) was injected into the vitreous space. One week later, each eye was enucleated and transverse sections were subjected to morphometric analysis. Enzyme-linked immunosorbent assay (ELISA) was conducted for the determination of IL-1 beta levels in retina. Immunohistochemical and immunoblot studies were also performed. In eyes that received an intravitreal injection of IL-1 beta (0.1 to 10 ng/eye), significant thinning of the inner plexiform layer (IPL) was observed (P
