Complete nucleotide sequence of the fusolin gene of an entomopoxvirus in the cupreous chafer, Anomala cuprea Hope (Coleoptera: Scarabaeidae).
Complete nucleotide sequence of the fusolin gene of an entomopoxvirus in the cupreous chafer, Anomala cuprea Hope (Coleoptera: Scarabaeidae).
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铜金龟子 Anomala cuprea Hope（鞘翅目：金龟子科）昆虫痘病毒的 Fusolin 基因的完整核苷酸序列。
DOI：
10.1016/s0965-1748(97)00069-6
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发表时间：
1997
影响因子：
3.8
通讯作者：
M. Sato
中科院分区：
文献类型：
--
作者：
W. Mitsuhashi;H. Saito;M. Sato
克隆了铜雀异蛾昆虫痘病毒（Anomala cuprea entomopoxvirus，AcEPV）的fusolin基因，并对其进行了序列测定。将该序列与已报道的其它昆虫EPVs如Melolontha melolontha（MmEPV）、Choristoneura biennis（CbEPV）和Heliothis armigera（HaEPV）的fusolin基因序列进行了比较。该基因具有一个1119 nt的开放阅读框（ORF），编码43.3kDa的蛋白质。蛋白质的微测序显示16个氨基酸残基的信号肽的切割。预测的氨基酸序列与其它EPVs中相应的氨基酸序列具有很高的同源性，与MmEPV、CbEPV和HaEPV的氨基酸同源性分别为53%、51%和52%。在ORF中，与其它EPV一样，检测到Vialard等（1990，Journal of Virology 64，5804-5811）描述的5个“保守区”。还检测到11个半胱氨酸残基，推测参与了次晶形成。另一方面，属于A属的AcEPV与属于B属的CbEPV和HaEPV一样，在ORF中具有潜在的N-糖基化位点，然而，在属于AcEPV的同一属的MmEPV中未检测到该位点。该ORF的最后330 bp区域与MmEPV的同源性较低。
The fusolin gene of Anomala cuprea entomopoxvirus (AcEPV) was cloned and sequenced. The sequence was compared with that of fusolin genes of EPVs from other insects such as Melolontha melolontha (MmEPV), Choristoneura biennis (CbEPV) and Heliothis armigera (HaEPV) previously reported. The gene harbored a single open reading frame (ORF) of 1119nt capable of coding for a protein of 43.3kDa. The microsequencing of the protein showed the cleavage of a signal peptide of 16 amino acid (aa) residues. The predicted aa sequence revealed significant homologies with the corresponding sequence in other EPVs, showing a 53, 51 and 52% aa-identity with MmEPV, CbEPV and HaEPV, respectively. In the ORF, five `conserved regions' described by Vialard et al. (1990, Journal of Virology 64, 5804–5811) like other EPVs were detected. Eleven cysteine residues, presumably involved in paracrystal formation, were also detected. On the other hand, AcEPV, belonging to Genus A, harbored a potential N-glycosylation site in the ORF like CbEPV and HaEPV belonging to Genus B which was not, however, detected in MmEPV belonging to the same Genus as AcEPV. Furthermore, the last 330bp region of the ORF revealed a low homology with that of MmEPV.
编码 34.8K 多肽的苜蓿银纹夜蛾核多角体病毒晚期基因的序列、转录和翻译。
DOI：
10.1099/0022-1317-70-9-2449
发表时间：
1989
期刊：
The Journal of general virology
影响因子：
--
作者：
Wu,JG;Miller,LK
通讯作者：
Miller,LK
Choristoneura 昆虫痘病毒包涵体内 Amsacta 河豚精蛋白样蛋白的鉴定。
影响因子：
3.7
作者：
Hall,RL;Moyer,RW
通讯作者：
Moyer,RW