CLD1/SRL1 modulates leaf rolling by affecting cell wall formation, epidermis integrity and water homeostasis in rice
CLD1/SRL1 modulates leaf rolling by affecting cell wall formation, epidermis integrity and water homeostasis in rice
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CLD1/SRL1 通过影响水稻细胞壁形成、表皮完整性和水稳态来调节叶片卷曲
DOI：
10.1111/tpj.13728
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发表时间：
2017-12-01
期刊：
影响因子：
7.2
通讯作者：
Chen, Kun-Ming
中科院分区：
文献类型：
--
作者：
Li, Wen-Qiang;Zhang, Min-Juan;Chen, Kun-Ming
卷叶性状是水稻育种的重要农艺性状之一。先前已经报道，半卷叶1（SRL 1）通过调节泡状细胞的形成来调节水稻（Oryza sativa）的卷叶;然而，SRL 1的调节机制尚未被进一步阐明。在这里，我们报告的功能特性的一种新的卷叶突变体，卷曲叶和侏儒1（cld 1），具有多种形态缺陷。图位克隆结果表明，CLD 1与SRL 1等位，CLD 1通过DNA甲基化而丧失功能。CLD 1/SRL 1编码糖磷脂酰肌醇（GPI）锚定的膜蛋白，调节叶片卷曲和水稻生长发育的其他方面。cld 1突变体表现出显着降低纤维素和木质素含量在次生细胞壁的叶片，表明CLD 1/SRL 1功能的丧失影响细胞壁的形成。此外，CLD 1/SRL 1功能的丧失会导致叶表皮缺陷，例如泡状表皮细胞。cld 1叶片表皮的缺陷降低了叶片的保水能力，导致叶片水分亏缺，是叶片卷曲的主要原因。由于叶片失水更快，含水量更低，cld 1表现出较低的耐旱性。因此，CLD 1/SRL 1功能的丧失导致与细胞壁形成、角质层发育和水分胁迫相关的基因和蛋白质的异常表达。综上所述，这些发现表明，CLD 1/SRL 1在卷叶调节中的功能作用与维持细胞壁形成、表皮完整性和水稳态密切相关。
Leaf rolling is considered as one of the most important agronomic traits in rice breeding. It has been previously reported that SEMI-ROLLED LEAF 1 (SRL1) modulates leaf rolling by regulating the formation of bulliform cells in rice (Oryza sativa); however, the regulatory mechanism underlying SRL1 has yet to be further elucidated. Here, we report the functional characterization of a novel leaf-rolling mutant, curled leaf and dwarf 1 (cld1), with multiple morphological defects. Map-based cloning revealed that CLD1 is allelic with SRL1, and loses function in cld1 through DNA methylation. CLD1/SRL1 encodes a glycophosphatidylinositol (GPI)-anchored membrane protein that modulates leaf rolling and other aspects of rice growth and development. The cld1 mutant exhibits significant decreases in cellulose and lignin contents in secondary cell walls of leaves, indicating that the loss of function of CLD1/SRL1 affects cell wall formation. Furthermore, the loss of CLD1/SRL1 function leads to defective leaf epidermis such as bulliform-like epidermal cells. The defects in leaf epidermis decrease the water-retaining capacity and lead to water deficits in cld1 leaves, which contribute to the main cause of leaf rolling. As a result of the more rapid water loss and lower water content in leaves, cld1 exhibits reduced drought tolerance. Accordingly, the loss of CLD1/SRL1 function causes abnormal expression of genes and proteins associated with cell wall formation, cuticle development and water stress. Taken together, these findings suggest that the functional roles of CLD1/SRL1 in leaf-rolling regulation are closely related to the maintenance of cell wall formation, epidermal integrity and water homeostasis.