CYTOKINES IN SYMPTOMATIC ASTHMA AIRWAYS
CYTOKINES IN SYMPTOMATIC ASTHMA AIRWAYS
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DOI：
10.1016/0091-6749(92)90218-q
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发表时间：
1992-05-01
影响因子：
14.2
通讯作者：
WASSERMAN, SI
中科院分区：
文献类型：
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作者：
BROIDE, DH;LOTZ, M;WASSERMAN, SI
为了确定哮喘患者体内是否产生细胞因子，我们用免疫分析法检测了24例有症状哮喘患者和9例无症状哮喘患者的呼吸道细胞因子(肿瘤坏死因子、粒细胞巨噬细胞集落刺激因子、白介素1α、白介素1α、白介素1β、白介素2、白介素4、白介素6)的水平。和IL-4)或生物测定(肿瘤坏死因子和白介素6)和聚合酶链式反应(白介素1-β和肿瘤坏死因子)。与无症状性哮喘患者相比，有症状哮喘患者的支气管肺泡灌洗液(BALF)中可检测到明显的肿瘤坏死因子(578+/-917pg/ml对24+/-29pg/ml)(p=0.01)、GM-CSF(24+/-41pg/ml对8 pg/ml)(p=0.02)和IL-6(225+/-327pg/ml对7+/-12pg/ml)(p=0.01)，但未检测到IL-1-α或IL-4。有症状哮喘患者BALF中IL-1-β水平(266pg/mlvs20pg/ml)和IL-2水平(1.4pg/mlvs0.3 ng/mlvs20pg/ml)(p=0.001)提示肺泡巨噬细胞和T细胞活化。因此，在哮喘发作时，可以在BALF中检测到几种细胞因子，包括肿瘤坏死因子、GM-CSF、IL-1-β、IL-2和IL-6。
To determine whether cytokines are generated in vivo in subjects with asthma, we have measured cytokine levels (tumor necrosis factor [TNF], granulocyte-macrophage-colony-stimulating factor [GM-CSF], interleukin [IL]-1-alpha, IL-1-beta, IL-2, IL-4, and IL-6) in the airways of subjects with symptomatic (N = 24) and asymptomatic (N = 9) asthma with immunoassays (GM-CSF, IL-1-alpha, IL-1-beta, IL-2, and IL-4) or bioassays (TNF and IL-6) and the polymerase chain reaction (IL-1-beta and TNF). Significant levels of TNF (578 +/- 917 pg/ml versus 24 +/- 29 pg/ml) (p = 0.01), GM-CSF (24 +/- 41 pg/ml versus < 8 pg/ml) (p = 0.02), and IL-6 (225 +/- 327 pg/ml versus 7 +/- 12 pg/ml) (p = 0.01), but not IL-1-alpha or IL-4, were detected in the bronchoalveolar lavage fluid (BALF) of patients with symptomatic compared with BALF of patients with asymptomatic asthma. Levels of IL-1-beta (266 +/- 270 pg/ml versus < 20 pg/ml) (p = 0.001) and IL-2 (1.4 +/- 2.8 ng/ml versus < 0.3 ng/ml) (p = 0.05) in BALF in patients with symptomatic compared with that in BALF levels in patients with asymptomatic asthma suggested activation of alveolar macrophages and T cells. Thus, in episodes of asthma, several cytokines, including TNF, GM-CSF, IL-1-beta, IL-2, and IL-6 are detectable in BALF.