Effect of prolonged intermittent hypoxia and exercise training on glucose tolerance and muscle GLUT4 protein expression in rats
Effect of prolonged intermittent hypoxia and exercise training on glucose tolerance and muscle GLUT4 protein expression in rats
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DOI：
10.1159/000081831
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发表时间：
2004-01-01
影响因子：
11
通讯作者：
Kuo, CH
中科院分区：
文献类型：
--
作者：
Chiu, LL;Chou, SW;Kuo, CH
比较了慢性间歇性低氧和耐力训练对大鼠骨骼肌糖耐量和GLUT 4蛋白表达的影响。将32只Sprague-Dawley大鼠的体重匹配，并分配到以下四组之一：对照组、耐力训练组、低氧组或低氧后进行耐力训练组。低氧治疗包括在常压条件下呼吸14%O-2 12 h/d，训练方案包括使动物游泳2次，每次3 h/d。第3周末，于治疗后16 h进行口服葡萄糖耐量试验（OGTT）。在第4周末，测定骨骼肌中GLUT 4蛋白、mRNA和糖原储存。耐力训练显著改善OGTT结果。糖原含量和GLUT 4蛋白表达在跖肌和红色腓肠肌，但不是在比目鱼肌或白色腓肠肌，也升高。慢性间歇性缺氧也改善OGTT结果，但不改变GLUT 4蛋白表达。此外，与单独进行耐力训练相比，低氧后进行运动训练在更多肌肉中产生了GLUT 4蛋白和mRNA的显着增加。无论是运动训练和缺氧显着降低体重，和两种治疗的累加效应被发现。总之，慢性间歇性缺氧改善葡萄糖耐量增加GLUT 4蛋白表达的情况下。这种处理促进了运动训练对肌肉GLUT 4表达和糖原储存的影响。这些新的发现开启了利用间歇性低氧（有或没有运动训练）预防和临床治疗2型糖尿病或胰岛素抵抗的可能性。版权所有（C）2004国家科学理事会，中华民国和S. Karger AG，巴塞尔。
We compared the chronic effect of intermittent hypoxia and endurance training on the glucose tolerance and GLUT4 protein expression in rat skeletal muscle. Thirty-two Sprague-Dawley rats were matched for weight and assigned to one of the following four groups: control, endurance training, hypoxia, or hypoxia followed by endurance training. Hypoxic treatment consisted of breathing 14% O-2 for 12 h/day under normobaric conditions, and the training protocol consisted of making animals swim 2 times for 3 h/day. At the end of the 3rd week, an oral glucose tolerance test (OGTT) was performed 16 h after treatments. At the end of the 4th week, GLUT4 protein, mRNA, and glycogen storage in skeletal muscle were determined. Endurance training significantly improved OGTT results. Glycogen content and GLUT4 protein expression in the plantaris and red gastrocnemius, but not in the soleus or white gastrocnemius muscles, were also elevated. Chronic intermittent hypoxia also improved OGTT results, but did not alter GLUT4 protein expression. Additionally, hypoxia followed by exercise training produced significant increases in GLUT4 protein and mRNA in a greater number of muscles compared to endurance training alone. Both exercise training and hypoxia significantly reduced body mass, and an additive effect of both treatments was found. In conclusion, chronic intermittent hypoxia improved glucose tolerance in the absence of increased GLUT4 protein expression. This treatment facilitated the exercise training effect on muscle GLUT4 expression and glycogen storage. These new findings open the possibility of utilizing intermittent hypoxia, with or without exercise training, for the prevention and clinical treatment of type 2 diabetes or insulin resistance. Copyright (C) 2004 National Science Council, ROC and S. Karger AG, Basel.