ULTRAFAST SEQUENCING OF OLIGODEOXYRIBONUCLEOTIDES BY FAB-MASS SPECTROMETRY
ULTRAFAST SEQUENCING OF OLIGODEOXYRIBONUCLEOTIDES BY FAB-MASS SPECTROMETRY
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DOI：
10.1093/nar/10.15.4671
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发表时间：
1982-01-01
影响因子：
14.9
通讯作者：
BLOCKER, H
中科院分区：
文献类型：
--
作者：
GROTJAHN, L;FRANK, R;BLOCKER, H
描述了一种用于寡脱氧核糖核苷酸双向测序的全仪器方法。该方法利用快原子轰击质谱的负离子碎裂模式。它比迄今为止已知的任何其他测序程序耗时更少。由于一次测序运行仅需1小时，因此预计这种新方法将大大减少（目前）长度高达10个核苷酸单位的寡脱氧核糖核苷酸的受控合成所需的时间。
A fully instrumental method is described for the bidirectional sequencing of oligodeoxyribonucleotides. The methcd makes use of the negative ion fragmentation patterns of fast atom bombardment mass spectrometry. It is less time consuming than any other sequencing procedure known to date. Since one sequencing run takes as little as one hour, this new method is anticipated to cut down considerably the time required for the controlled synthesis of oligodeoxyribonucleotides of (currently) up to ten nucleotide units in length.