Both genes for EF-1 alpha in Candida albicans are translated.
Both genes for EF-1 alpha in Candida albicans are translated.
复制标题
白色念珠菌中 EF-1 α 的两个基因均被翻译。
DOI：
10.1111/j.1365-2958.1991.tb01918.x
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发表时间：
1991
影响因子：
3.6
通讯作者：
Sypherd,PS
中科院分区：
文献类型：
--
作者：
Sundstrom,P;Irwin,M;Smith,D;Sypherd,PS
在以前的工作中，我们发现白色念珠菌有两个基因TEF-1和TEF-2，它们编码高度保守的必需蛋白质合成因子EF-1α的相同多肽（Breviarioet al.，1988年）。这一结果引发了关于C. albicanspreferentially使用其中一个基因在其他和是否两个基因实际上被翻译成蛋白质。每个基因的非翻译部分的基因特异性序列差异使得可以制备基因特异性寡核苷酸杂交探针。探针的结果表明，两种基因的相对稳态mRNA水平相当，并且每个基因的mRNA都存在于活性翻译复合物中。
In previous work, we showed thatCandida albicanshas two genes,TEF‐1andTEF‐2, which encode identical polypeptides for the highly conserved, essential, protein synthesis factor EF‐1α (Breviarioet al., 1988). This result prompted questions as to whetherC. albicanspreferentially uses one of the genes over the other and whether both genes are actually translated into protein. Gene‐specific sequence differences in the untranslated portion of each gene made it possible to prepare gene‐specific oligonucleotide hybridization probes. Results with the probes showed that the relative steady‐state mRNA levels of the two genes were equivalent and that the mRNA for each gene was present in active translation complexes.