Leucine and glutamine metabolism in septic rats.
Leucine and glutamine metabolism in septic rats.
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脓毒症大鼠的亮氨酸和谷氨酰胺代谢。
DOI：
10.1042/bj2760405
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发表时间：
1991
期刊：
影响因子：
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通讯作者：
Stein,TP
中科院分区：
文献类型：
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作者：
Yoshida,S;Lanza-Jacoby,S;Stein,TP
比较正常大鼠和脓毒症大鼠亮氨酸C-2掺入谷氨酰胺的速率。雌性SD大鼠46只，体重210~260g，经胃肠外喂养3d后随机分为对照组和脓毒症组。脓毒症组于第4天注射10(10)活菌/kg建立脓毒症模型。每组大鼠均连续输注三种不同同位素中的一种(8h)。接种后24小时给药。[1-13C]亮氨酸测定蛋白质的亮氨酸氧化和掺入，[5-13C]谷氨酰胺测定谷氨酰胺通量和氧化，[1，2-13C]亮氨酸测定[1，2-13C]亮氨酸C-2掺入谷氨酰胺的比例。结果表明：脓毒症使亮氨酸C-2掺入谷氨酰胺的速率显著增加(66.0+/-3.7比29.6+/-3.7 Mumol/h，P<0.01)。这一增长既是由于谷氨酰胺产量的增加(2331+/-76比1959+/-94摩尔/小时每公斤，P<0.01)，也是由于来自亮氨酸的谷氨酰胺比例增加(2.83+/-0.27%比1.51+/-0.31%，P<0.01)。脓毒症时谷氨酰胺结合的亮氨酸C-2与氧化亮氨酸的比值由7.16+/-0.91%升至11.49+/-1.12%(P<0.05)。
The rate of leucine C-2 incorporation into glutamine was compared in control and septic rats. Female Sprague-Dawley rats (n = 46, 210-260 g) were fed parenterally for 3 days and then randomized into two groups (control and septic). Sepsis was induced by the injection of 10(10) live Escherichia coli/kg on day 4 into the septic group. Rats in each group were given a continuous (8 h) infusion of one of three different isotopes. The isotopes were given 24 h after inoculation. Leucine oxidation and incorporation into protein were determined with [1-13C]leucine; glutamine flux and oxidation were determined with [5-13C]glutamine, and the fraction of leucine C-2 incorporated into glutamine was determined by giving [1,2-13C]leucine. Results were as follows: sepsis caused a significant increase in the rate of leucine C-2 incorporation into glutamine (66.0 +/- 3.7 as against 29.6 +/- 3.7 mumol/h per kg, P less than 0.01). This increase was due to both an increase in glutamine production (2331 +/- 76 as against 1959 +/- 94 mumol/h per kg, P less than 0.01) and an increase in the proportion of glutamine derived from leucine (2.83 +/- 0.27% as against 1.51 +/- 0.31%, P less than 0.01). The ratio of leucine C-2 incorporated into glutamine to leucine oxidized increased from 7.16 +/- 0.91% to 11.49 +/- 1.12% with sepsis (P less than 0.05).