Phylogenomics solves a long-standing evolutionary puzzle in the ciliate world: The subclass Peritrichia is monophyletic
Phylogenomics solves a long-standing evolutionary puzzle in the ciliate world: The subclass Peritrichia is monophyletic
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DOI：
10.1016/j.ympev.2016.09.016
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发表时间：
2017-01-01
影响因子：
4.1
通讯作者：
Lynn, Denis
中科院分区：
文献类型：
--
作者：
Gentekaki, Eleni;Kolisko, Martin;Lynn, Denis
纤毛虫门是研究最广泛的原生动物谱系之一。分子研究的时代带来了一个重大的持续争论：1859年的Peritrichia Stein亚类是单系的吗？许多主要使用小亚单位（SSU） rRNA基因的分析都未能恢复Mobilida和Sessilida这两个富有的目作为姐妹分支。在这里，我们已经测序了5个富含元素的物种- 3个无柄类和2个流动类。我们构建了24种纤毛虫的158个基因和44,696个性状的超矩阵，作为外群分类群，有9种来自顶复亚，4种来自甲藻亚。我们的分析使用最大似然和贝叶斯方法恢复单系类寡膜蜂和其中的两个稳健的分支。第一个Glade是一个单系的褐枝属，其中Sessilida目和Mobilida目作为姐妹分支得到了最大的支持。第二类少膜林包括膜孔虫亚纲和膜孔虫亚纲，它们是姐妹分支。我们的分析解决了长期以来在纤毛虫分子系统发育上的争论，并为“Mobilida和Sessilida两种富缘目的形态特征来自同一个共同祖先而不是趋同的结果”的经典观点提供了支持。(C) 2016 Elsevier Inc.版权所有。
The phylum Ciliophora is one of the most broadly studied protozoan lineages. The era of molecular investigation has brought forth a major ongoing debate: is the subclass Peritrichia Stein, 1859 monophyletic? Numerous analyses mostly using the small subunit (SSU) rRNA gene have failed to recover the Mobilida and Sessilida, the two peritrich orders, as sister clades. Here we have sequenced five peritrich species - three sessilids and two mobilids. We constructed a supermatrix of 158 genes and 44,696 characters for 24 ciliate species, and as outgroup taxa, nine species from the Apicomplexa and four from the Dinophyceae. Our analyses using both maximum likelihood and Bayesian methods recover a monophyletic class Oligohymenophorea and two robust clades within it. The first Glade is a monophyletic Peritrichia with the orders Sessilida and Mobilida maximally supported as sister clades. The second oligohymenophorean Glade includes species of the subclasses Scuticociliatia and Hymenostomatia, which are sister clades. Our analyses resolve a long-standing debate in ciliate molecular phylogenetics and provide support for the classical view that the morphological features of the two peritrich orders Mobilida and Sessilida arose by descent from the same common ancestor and are not the result of convergence. (C) 2016 Elsevier Inc. All rights reserved.