The CADM2/Akt pathway is involved in the inhibitory effect of miR-21-5p downregulation on proliferation and apoptosis in esophageal squamous cell carcinoma cells
The CADM2/Akt pathway is involved in the inhibitory effect of miR-21-5p downregulation on proliferation and apoptosis in esophageal squamous cell carcinoma cells
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CADM 2/Akt通路参与miR-21- 5 p下调抑制食管鳞癌细胞增殖和凋亡
DOI：
10.1016/j.cbi.2018.04.021
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发表时间：
2018-05-25
影响因子：
5.1
通讯作者：
Zhao, Hui
中科院分区：
文献类型：
--
作者：
Li, Xiaohui;Chen, Dong;Zhao, Hui
食管鳞状细胞癌（ESCC）是食管癌的主要亚型，是全球第八大常见癌症。细胞粘附分子2 （CADM2）是一种肿瘤抑制因子，在多种癌症中通常下调。然而，CADM2在ESCC中的作用尚不清楚。本研究的目的是评估CADM2在ESCC中的潜在作用和潜在的作用机制。在此，我们发现CADM2在ESCC组织和细胞系中低表达。CADM2过表达抑制ESCC细胞增殖，诱导细胞凋亡。此外，CADM2过表达也抑制了ESCC细胞中Akt信号通路。MiR-21-5p下调可抑制细胞增殖，诱导细胞凋亡，而CADM2下调可减弱抗MiR-21-5p的作用。在转染anti-miR-21的细胞中，p-Akt的表达降低。然而，与转染anti-miR-21-5p的细胞相比，共转染anti-miR-21-5p和si-CADM2的细胞中p-Akt的表达增加。综上所述，CADM2/Akt通路参与了miR-21-5p下调对ESCC细胞增殖和凋亡的抑制作用。这些发现表明miR-21-5p/CADM2/Akt轴可能是治疗ESCC的新途径。
Esophageal squamous cell carcinoma (ESCC), the main subtype of esophageal cancer, is the eighth most common cancer worldwide. Cell adhesion molecule 2 (CADM2) has been reported to be a tumor suppressor and is usually downregulated in several cancers. However, the role of CADM2 in ESCC remains unknown. The aim of the present study was to evaluate the potential role and underlying action mechanism of CADM2 in ESCC. Herein, we found that CADM2 was low-expressed in ESCC tissues and cell lines. CADM2 overexpression inhibited proliferation and induced apoptosis of ESCC cells. Moreover, CADM2 overexpression also suppressed the Akt signaling pathway in ESCC cells. MiR-21-5p down-regulation inhibited cell proliferation and induced cell apoptosis, while CADM2 knockdown attenuated the effect of anti-miR-21-5p. The expression of p-Akt was decreased in the cells transfected with anti-miR-21. However, the expression of p-Akt was increased in the cells co-transfected with anti-miR-21-5p and si-CADM2 compared with that in anti-miR-21-5p-transfecting cells. In summary, the CADM2/Akt pathway is involved in the inhibitory effect of miR-21-5p downregulation on proliferation and apoptosis in ESCC cells. These findings indicated that the miR-21-5p/CADM2/Akt axis might be a new approach for the treatment of ESCC.