Gene Expression of Growth Hormone Family and Glucocorticoid Receptors, Osmosensors, and Ion Transporters in the Gill During Seawater Acclimation of Mozambique Tilapia, Oreochromis mossambicus
Gene Expression of Growth Hormone Family and Glucocorticoid Receptors, Osmosensors, and Ion Transporters in the Gill During Seawater Acclimation of Mozambique Tilapia, Oreochromis mossambicus
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DOI：
10.1002/jez.613
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发表时间：
2010-08-01
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影响因子：
--
通讯作者：
Grau, E. Gordon
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文献类型：
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作者：
Breves, Jason P.;Fox, Bradley K.;Grau, E. Gordon
本研究的特点是内分泌和离子调节反应伴随着海水（SW）驯化莫桑比克罗非鱼（Oreochromis mossambicus）。测定了鳃中激素受体、推定的受体和离子转运蛋白的血浆激素和基因表达的变化。淡水（FW）驯化罗非鱼转移到SW导致血浆渗透压和血浆生长激素（GH）水平在12小时和14天后转移显着上升。血浆催乳素（PRL 177和PRL 188）水平显着减少也发生在SW转移鱼，没有影响后，血浆皮质醇或胰岛素样生长因子I转移观察。GH受体的基因表达在转移后6小时强烈增加，而PRL受体在12小时低于对照组。相反，生长激素和糖皮质激素受体的mRNA水平不受SW转移。渗透应激转录因子1 mRNA水平在3至12小时之间显着升高，而钙敏感受体则不受影响。水通道蛋白-3基因表达强烈下调SW驯化期间从12小时，直到实验结束。Na+/K+/2Cl（-）协同转运蛋白基因表达增加显着3小时后转移，而表达的Na+/Cl-协同转运蛋白，特定的RN型氯细胞，下降到SW驯化6小时。Na+/H+交换器的反应不太明显，但显示出类似的模式，Na+/Cl-共转运。这些结果表明，收购hyposmoregulatory机制在莫桑比克罗非鱼需要协调的相互作用，全身激素与当地的因素在鳃，包括激素受体，离子转运蛋白，和β-受体。J. Exp. Zool.313A：432-441，2010. (C)2010 Wiley-Liss，Inc.
This study characterized endocrine and ionoregulatory responses accompanying seawater (SW) acclimation in Mozambique tilapia (Oreochromis mossambicus). Changes in plasma hormones and gene expression of hormone receptors, putative osmosensors, and ion transporters in the gill were measured. Transfer of freshwater (FW)-acclimated tilapia to SW resulted in a marked elevation in plasma osmolality and a significant rise in plasma growth hormone (GH) levels at 12 hr and 14 days after transfer. Significant reductions in plasma prolactin (PRL177 and PRL188) levels also occurred in SW-transferred fish; no effect of transfer upon plasma cortisol or insulin-like growth factor I was observed. Gene expression of GH receptor increased strongly 6 hr after transfer, whereas PRL receptor was lower than controls at 12 hr. By contrast, mRNA levels of somatolactin and glucocorticoid receptors were unaffected by SW transfer. Osmotic stress transcription factor 1 mRNA levels rose significantly between 3 and 12 hr, whereas the calcium-sensing receptor was unaffected. Aquaporin-3 gene expression was strongly down-regulated during SW acclimation from 12 hr until the conclusion of the experiment. Na+/K+/2Cl(-) cotransporter gene expression increased significantly 3 hr after transfer, whereas expression of Na+/Cl- cotransporter, specific to RN-type chloride cells, declined by 6 hr into SW acclimation. The response of Na+/H+ exchanger was less pronounced, but showed a similar pattern to that of the Na+/Cl- cotransporter. These results suggest that acquisition of hyposmoregulatory mechanisms in Mozambique tilapia entails the coordinated interaction of systemic hormones with local factors in the gill, including hormone receptors, ion transporters, and osmosensors. J. Exp. Zool. 313A:432-441, 2010. (C) 2010 Wiley-Liss, Inc.