Molecular Phylogenetic Analysis of Orientia tsutsugamushi Based on the groES and groEL Genes
Molecular Phylogenetic Analysis of Orientia tsutsugamushi Based on the groES and groEL Genes
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DOI：
10.1089/vbz.2012.1155
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发表时间：
2013-11-01
影响因子：
2.1
通讯作者：
Oishi, Kazunori
中科院分区：
文献类型：
--
作者：
Arai, Satoru;Tabara, Kenji;Oishi, Kazunori
用PCR方法扩增恙虫病东方体GroES和GroEL蛋白的DNA序列并进行测序。两种基因全长序列的同源性分析表明，O.提示这些基因可用于恙虫病的分子诊断和系统发育分析。56 kD型特异性抗原（TSA）蛋白基因和groES和groEL基因的比较表明，基因型的基础上的56 kD TSA基因不相关的一个簇包含groES和groEL基因的树状图，这表明基因重排可能与螨的同源重组。
DNA sequences encoding the GroES and GroEL proteins of Orientia tsutsugamushi were amplified by the PCR and sequenced. Pairwise alignment of full-length groES and groEL gene sequences indicated high sequence similarity (90.4-100% and 90.3-100%) in O. tsutsugamushi, suggesting that these genes are good candidates for the molecular diagnosis and phylogenetic analysis of scrub typhus. Comparisons of the 56-kD type-specific antigen (TSA) protein gene and the groES and groEL genes showed that genotypes based on the 56-kD TSA gene were not related to a cluster containing the groES and groEL genes in a dendrogram, suggesting that a gene rearrangement may be associated with homologous recombination in mites.