The use of a semiocclusive dressing reduces epidermal inflammatory cytokine expression and mitigates dermal proliferation and inflammation in a rat incisional model.
The use of a semiocclusive dressing reduces epidermal inflammatory cytokine expression and mitigates dermal proliferation and inflammation in a rat incisional model.
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DOI：
10.1111/j.1524-475x.2008.00404.x
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发表时间：
2008-07
影响因子：
2.9
通讯作者：
Mustoe, Thomas A.
中科院分区：
文献类型：
--
作者：
Kloeters, Oliver;Schierle, Clark;Tandara, Andrea;Mustoe, Thomas A.
闭塞性伤口敷料在临床上用于加速伤口愈合和改善疤痕的最终外观。体内和体外证据表明，这种作用的一种机制是维持表皮的正常水合作用，尽管分子信号仍未被表征。我们试图在大鼠创伤半闭塞模型中阐明组织学变化和一些分子信号。我们采用手术带闭塞的大鼠线性切口模型。采用组织学染色和实时荧光定量PCR分析表征半封闭对创面愈合反应的细胞和分子影响。与未封闭的对照伤口相比，半封闭伤口表现出表皮厚度和细胞数量减少，有丝分裂表皮活性降低。相关的真皮细胞也同样因半封闭而减弱。最后，与未闭塞的伤口相比，术后第3天，促炎细胞因子il -1- α和tnf - α的转录水平显著降低。在大鼠切口伤口模型中，半封闭伤口处理显著降低表皮厚度、细胞数量、有丝分裂活性和真皮细胞数量以及重要的表皮炎症介质的转录水平。
Occlusive wound dressings are utilized clinically to accelerate wound healing and improve the final appearance of scars. In vivo and in vitro evidence suggests that one mechanism for this effect is maintenance of normal hydration in the epidermis, although the molecular signals remain uncharacterized. We sought to elucidate histological changes and some of the molecular signals involved in this effect in a rat model of wound semi-occlusion. We utilized a rat linear incision model with surgical tape occlusion. Histological stains and quantitative real-time PCR analysis were used to characterize the cellular and molecular effects of semi-occlusion on the wound healing response. Semi-occluded wounds demonstrated decreased epidermal thickness and cellularity and less mitotic epidermal activity when compared with non-occluded control wounds. Associated dermal cellularity was similarly attenuated by semi-occlusion. Finally, levels of proinflammatory cytokines IL-1-alpha and TNF-alpha were significantly decreased on postoperative day 3 at the transcriptional level when compared with non-occluded wounds. Semi-occlusive wound treatments significantly decrease epidermal thickness, cellularity, mitotic activity and dermal cellularity as well as transcriptional levels of important epidermal mediators of inflammation in a rat incisional wound model.