Nitric Oxide Mediated Transcriptome Profiling Reveals Activation of Multiple Regulatory Pathways in Arabidopsis thaliana.
Nitric Oxide Mediated Transcriptome Profiling Reveals Activation of Multiple Regulatory Pathways in Arabidopsis thaliana.
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DOI：
10.3389/fpls.2016.00975
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发表时间：
2016
影响因子：
5.6
通讯作者：
Yun BW
中科院分区：
文献类型：
--
作者：
Hussain A;Mun BG;Imran QM;Lee SU;Adamu TA;Shahid M;Kim KM;Yun BW
一氧化氮及其衍生物的积累和去除之间的不平衡是所有植物在细胞水平上面临的挑战，并且在胁迫条件下尤其重要。植物暴露于各种生物和非生物胁迫导致细胞氧化还原音的快速变化，活性氮物种的增加增强了细胞氧化还原音，活性氮物种作为介导防御反应的信号分子。为了了解这些信号分子介导的机制，我们进行了大规模的亚硝化胁迫诱导的拟南芥转录组分析。我们分别从对照和1 mM S-亚硝基半胱氨酸（CysNO）渗透的拟南芥叶样品的三个重复中产生了平均84和91百万个读数。比对后，超过95%的所有读数成功映射到参考，获得了32，535个基因和55，682个转录本。CysNO浸润后6 h，共检测到6436个基因（3448个上调，2988个下调）和6214个转录本（3335个上调，2879个下调）的差异表达。这些差异表达的基因被发现参与关键的生理过程，包括植物对各种生物和非生物胁迫的防御，激素信号传导和其他发育过程。在FPKM值的分位数标准化之后，接着进行学生T检验（P < 0.05），我们鉴定了1165个DEG（463个上调，702个下调），其在CysNO处理后表达变化至少2倍。使用定量实时PCR验证参与各种生物学途径的选定基因的表达模式。本研究提供了有关植物在转录水平上对亚硝化胁迫反应的全面信息，将有助于理解和整合植物亚硝化胁迫反应的相关机制。
Imbalance between the accumulation and removal of nitric oxide and its derivatives is a challenge faced by all plants at the cellular level, and is especially important under stress conditions. Exposure of plants to various biotic and abiotic stresses causes rapid changes in cellular redox tone potentiated by the rise in reactive nitrogen species that serve as signaling molecules in mediating defensive responses. To understand mechanisms mediated by these signaling molecules, we performed a large-scale analysis of the Arabidopsis transcriptome induced by nitrosative stress. We generated an average of 84 and 91 million reads from three replicates each of control and 1 mM S-nitrosocysteine (CysNO)-infiltrated Arabidopsis leaf samples, respectively. After alignment, more than 95% of all reads successfully mapped to the reference and 32,535 genes and 55,682 transcripts were obtained. CysNO infiltration caused differential expression of 6436 genes (3448 up-regulated and 2988 down-regulated) and 6214 transcripts (3335 up-regulated and 2879 down-regulated) 6 h post-infiltration. These differentially expressed genes were found to be involved in key physiological processes, including plant defense against various biotic and abiotic stresses, hormone signaling, and other developmental processes. After quantile normalization of the FPKM values followed by student's T-test (P < 0.05) we identified 1165 DEGs (463 up-regulated and 702 down-regulated) with at least 2-folds change in expression after CysNO treatment. Expression patterns of selected genes involved in various biological pathways were verified using quantitative real-time PCR. This study provides comprehensive information about plant responses to nitrosative stress at transcript level and would prove helpful in understanding and incorporating mechanisms associated with nitrosative stress responses in plants.