Apoptosis in pyrogallol-treated Calu-6 cells is correlated with the changes of intracellular GSH levels rather than ROS levels
Apoptosis in pyrogallol-treated Calu-6 cells is correlated with the changes of intracellular GSH levels rather than ROS levels
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DOI：
10.1016/j.lungcan.2007.08.034
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发表时间：
2008-03-01
期刊：
影响因子：
5.3
通讯作者：
Park, Woo Hyun
中科院分区：
文献类型：
--
作者：
Han, Yong Hwan;Kim, Sung Zoo;Park, Woo Hyun
我们研究了参与谷胱甘肽（GSH）和活性氧（ROS），如H2 O2和O-2（-中心点）在焦性没食子酚处理的Calu-6细胞的死亡。邻苯三酚抑制Calu-6细胞的生长，IC 50约为50 μ M。邻苯三酚处理Calu-6细胞72 h后，细胞内H2 O2水平没有改变或降低。然而，O-2水平（-中心点）增加。连苯三酚处理也降低了细胞内GSH含量。邻苯三酚处理72 h后，SOD活性下降，而过氧化氢酶活性上升。ROS清除剂，包括Tempol，Tiron，曲美他嗪和N-乙酰半胱氨酸（NAC），没有降低细胞内O-2的水平（中心点-）。坦波尔表明在连苯三酚处理的细胞中GSH消耗的恢复显著防止了凋亡，而Tiron防止了线粒体跨膜电位（Δ Psi（m））的丧失。与此相反，NAC治疗显示O-2（中心点-）水平和GSH耗竭的影响增加，加强焦性没食子酚诱导的细胞凋亡。此外，用SOD和过氧化氢酶处理显著防止焦性没食子酚处理的Calu-6细胞中线粒体跨膜电位（Delta Psi（m））的丢失。然而，只有过氧化氢酶显示O-2（-中心点）水平和GSH耗尽的影响降低防止焦性没食子酚诱导的细胞凋亡。总之，焦性没食子酚处理的Calu-6细胞的凋亡与细胞内GSH水平的变化相关，而不是ROS水平。(C)2007爱思唯尔爱尔兰有限公司保留所有权利。
We investigated the involvement of glutathione (GSH) and reactive oxygen species (ROS) such as H2O2 and O-2(-center dot) in the deaths of pyrogallol-treated Calu-6 cells. Pyrogallol inhibited the growth of Calu-6 cells with an IC50 of approximately 50 mu M. Levels of intracellular H2O2 were not altered or were decreased in pyrogallol-treated Calu-6 cells at 72 h. However, levels Of O-2(-center dot) were increased. Treatment with pyrogallol also reduced the intracellular GSH content. The activity of SOD was down-regulated, but the activity of catalase was up-regulated by pyrogallol at 72 h. ROS scavengers, including Tempol, Tiron, Trimetazidine, and N-acetylcysteine (NAC), did not reduce the levels of the intraceltular O-2(center dot-). Tempol. showing the recovery of GSH depletion in pyrogallol-treated cells significantly prevented apoptosis, while Tiron prevented the loss of mitochondrial transmembrane potential (Delta Psi(m)). In contrast, treatment with NAC showing an increased effect on O-2(center dot-) levels and depletion of GSH intensified pyrogallol-induced apoptosis. In addition, treatment with SOD and catalase significantly prevented the toss of mitochondrial transmembrane potential (Delta Psi(m)) in pyrogallol-treated Calu-6 cells. However, only catalase showing a decreased effect on O-2(-center dot) levels and depletion of GSH prevented pyrogallol-induced apoptosis. Taken together, apoptosis in pyrogallol-treated Calu-6 cells is correlated with the changes of intracellular GSH levels rather than ROS levels. (C) 2007 Elsevier Ireland Ltd. All rights reserved.