Enumeration and isolation of cellulolytic and hemicellulolytic bacteria from human feces
Enumeration and isolation of cellulolytic and hemicellulolytic bacteria from human feces
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人类粪便中纤维素分解菌和半纤维素分解菌的计数和分离
DOI：
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发表时间：
1988
影响因子：
4.4
通讯作者：
L. Montgomery
中科院分区：
文献类型：
--
作者：
Karen J. Wedekind;Howard R. Mansfield;L. Montgomery
对人大肠的纤维分解微生物区系进行了检测，以确定存在的纤维分解细菌和半纤维分解细菌的数量和类型。五个人的粪便样本中都含有能够降解经碱性过氧化氢(AHP-WS)处理的菠菜和麦草中的水合纤维素的细菌，而五个样本中只有一个样本检测到Whatman 1号滤纸(PMC)中相对结晶的纤维素被降解。以AHP-WS为底物估算的纤维分解菌平均浓度为1.2×10(8)/ml粪便。在AHP-WS上分离的细菌的纯培养物能够降解PMC，这表明与其他微生物的相互作用是以前以PMC为底物的粪便纤维分解菌检测成功率低的主要原因。纤维分解菌包括瘤胃球菌属、梭状芽胞杆菌属和两株未鉴定菌株。以落叶松木聚糖为底物，半纤维素细菌的平均浓度为1.8×10(10)/ml粪便。降解半纤维素的细菌包括丁状弧菌、梭状芽孢杆菌、类杆菌和两株未鉴定的菌株，以及5株纤维素分解菌中的4株。这项工作表明，许多人类拥有肠道纤维分解细菌，而水合纤维素源，如AHP-WS，对于它们的一致检测和分离是必要的。
The fibrolytic microbiota of the human large intestine was examined to determine the numbers and types of cellulolytic and hemicellulolytic bacteria present. Fecal samples from each of five individuals contained bacteria capable of degrading the hydrated cellulose in spinach and in wheat straw pretreated with alkaline hydrogen peroxide (AHP-WS), whereas degradation of the relatively crystalline cellulose in Whatman no. 1 filter paper (PMC) was detected for only one of the five samples. The mean concentration of cellulolytic bacteria, estimated with AHP-WS as a substrate, was 1.2 X 10(8)/ml of feces. Pure cultures of bacteria isolated on AHP-WS were able to degrade PMC, indicating that interactions with other microbes were primarily responsible for previous low success rates in detecting fecal cellulolytic bacteria with PMC as a substrate. The cellulolytic bacteria included Ruminococcus spp., Clostridium sp., and two unidentified strains. The mean concentration of hemicellulolytic bacteria, estimated with larchwood xylan as a substrate, was 1.8 X 10(10)/ml of feces. The hemicellulose-degrading bacteria included Butyrivibrio sp., Clostridium sp., Bacteroides sp., and two unidentified strains, as well as four of the five cellulolytic strains. This work demonstrates that many humans harbor intestinal cellulolytic bacteria and that a hydrated cellulose source such as AHP-WS is necessary for their consistent detection and isolation.
中性洗涤纤维、半纤维素和纤维素在人体中的消化率。
DOI：
10.1093/jn/111.2.287
发表时间：
1981
期刊：
The Journal of nutrition
影响因子：
--
作者：
Slavin,JL;Brauer,PM;Marlett,JA
通讯作者：
Marlett,JA