Two-Dimensional Difference Gel Electrophoresis (DiGE) Analysis of Plasmas from Dengue Fever Patients
Two-Dimensional Difference Gel Electrophoresis (DiGE) Analysis of Plasmas from Dengue Fever Patients
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DOI：
10.1021/pr900236f
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发表时间：
2009-12-01
影响因子：
4.4
通讯作者：
Neves-Ferreira, Ana G. C.
中科院分区：
文献类型：
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作者：
Albuquerque, Lidiane M.;Trugilho, Monique R. O.;Neves-Ferreira, Ana G. C.
登革热是世界上影响人类的最重要的节肢动物传播的病毒性疾病。为了更好地了解其发病机制，本研究旨在鉴定重症登革热患者血浆中与健康献血员相比差异表达的蛋白质，利用二维荧光差异凝胶电泳分析去除6种高丰度蛋白质的血浆结果表明，用蛋白质组学方法检测到73个差异表达的蛋白质点，其中蛋白质组学方法检测到的蛋白质点有：白蛋白、IgG、抗胰蛋白酶、伊加、转铁蛋白和触珠蛋白（n = 13，p < 0.01），其中37个可通过质谱鉴定。这37个点共包含14种蛋白质，如下所示：7个在登革热患者血浆中表达增加（C1抑制剂、α 1-抗胰凝乳蛋白酶、维生素D结合蛋白、纤维蛋白原γ链、α 1-酸性糖蛋白、载脂蛋白J和补体成分C3 c），而其他7种在相同样本中表达降低（α-2巨球蛋白、凝血酶原、富组氨酸糖蛋白、载脂蛋白A-IV和A-I、甲状腺素运载蛋白和补体成分C3 b）。在这项研究中，这些蛋白质可能参与登革热病毒感染引发的炎症过程和血管损伤的修复机制发生在这种病理进行了讨论。
Dengue fever is the world's most important arthropod-born viral disease affecting humans. To contribute to a better understanding of its pathogenesis, this study aims to identify proteins differentially expressed in plasmas from severe dengue fever patients relative to healthy donors, The use of 2-D Fluorescence Difference Gel Electrophoresis to analyze plasmas depleted of six high-abundance proteins (albumin, IgG, antitrypsin, IgA, transferrin and haptoglobin) allowed for the detection of 73 differentially expressed protein spots (n = 13, p < 0.01), of which 37 could be identified by mass spectrometry. These 37 spots comprised a total of 14 proteins, as follows: 7 had increased expression in plasmas from dengue fever patients (C1 inhibitor, alpha 1-antichymotrypsin, vitamin D-binding protein, fibrinogen gamma-chain, alpha 1-acid glycoprotein, apolipoprotein J and complement component C3c), while 7 others had decreased expression in the same samples (alpha-2 macroglobulin, prothrombin, histidine-rich glycoprotein, apolipoproteins A-IV and A-I, transthyretin and complement component C3b). The possible involvement of these proteins in the inflammatory process triggered by dengue virus infection and in the repair mechanisms of vascular damage occurring in this pathology is discussed in this study.