Direct assembling methodologies for high-throughput bioscreening.
Direct assembling methodologies for high-throughput bioscreening.
复制标题
用于高通量生物筛选的直接组装方法。
DOI：
10.1002/biot.201100100
复制
发表时间：
2011
影响因子：
4.7
通讯作者：
Xu,Tao
中科院分区：
文献类型：
--
作者：
Rodriguez-Devora,JorgeI;Shi,Zhi-dong;Xu,Tao
在过去的几十年里，高通量（HT）生物筛选是一种能够针对生物靶标快速筛选生化化合物库的技术，已广泛应用于药物发现、干细胞研究、新生物材料的开发和基因组学研究。为了实现这些目标，感兴趣的生物实体的无支架（或直接）组装变得至关重要。构建高效的 HT 生物筛选平台需要适当的组装方法。接触式和非接触式组装系统作为实用解决方案的发展受到生物筛选系统的各种基本属性的推动，例如小型化、高通量和高精度。本文综述了用于构建 HT 生物筛选平台的组装技术的最新进展。
Over the last few decades, high‐throughput (HT) bioscreening, a technique that allows rapid screening of biochemical compound libraries against biological targets, has been widely used in drug discovery, stem cell research, development of new biomaterials, and genomics research. To achieve these ambitions, scaffold‐free (or direct) assembly of biological entities of interest has become critical. Appropriate assembling methodologies are required to build an efficient HT bioscreening platform. The development of contact and non‐contact assembling systems as a practical solution has been driven by a variety of essential attributes of the bioscreening system, such as miniaturization, high throughput, and high precision. The present article reviews recent progress on these assembling technologies utilized for the construction of HT bioscreening platforms.