Bulbar acetylcholine enhances neural and perceptual odor discrimination.
Bulbar acetylcholine enhances neural and perceptual odor discrimination.
复制标题
延髓乙酰胆碱增强神经和感知气味辨别能力。
DOI：
10.1523/jneurosci.4036-08.2009
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发表时间：
2009-01-07
期刊：
影响因子：
--
通讯作者：
Linster C
中科院分区：
文献类型：
--
作者：
Chaudhury D;Escanilla O;Linster C
实验和建模数据表明，主嗅球（OB）的电路在嗅觉辨别中起着至关重要的作用。这些信息的处理源于OB输出神经元与局部中间神经元之间的相互作用，以及OB网络与离心输入之间的相互作用。特别是胆碱能输入到OB已被假设为调节二尖瓣细胞气味感受野（ORF）和类似气味的行为歧视。我们记录了麻醉大鼠OB中单个二尖瓣细胞，以确定暴露于气味刺激后单个二尖瓣细胞ORF的重叠程度。通过加入抗胆碱酯酶药物新斯的明（20 mM）来增加OB中胆碱能神经传递的功效，使二尖瓣细胞的嗅觉感受野（ORF）反应变得尖锐。此外，共同加入烟碱拮抗剂MLA（20 mM）或毒蕈碱拮抗剂东莨菪碱（40 mM）与新斯的明（20 mM）减弱了新斯的明依赖的ORF的锐化。这些电生理结果是预测伴随的行为实验中，胆碱能调制操作直接注入新斯的明，MLA和东莨菪碱到OB在嗅觉行为任务。在这些实验中，增加OB中胆碱能作用的功效增加了对气味的感知辨别，而阻断烟碱或毒蕈碱受体则降低了感知辨别。这些实验表明，行为歧视调制的方式预测的变化，在二尖瓣细胞的开放阅读框胆碱能药物。这些结果一起提出了第一个直接比较神经和知觉的影响延髓神经调质。
Experimental and modeling data suggest that the circuitry of the main olfactory bulb (OB) plays a critical role in olfactory discrimination. Processing of such information arises from the interaction between OB output neurons local interneurons, as well as interactions between the OB network and centrifugal inputs. Cholinergic input to the OB in particular has been hypothesized to regulate mitral cell odorants receptive fields (ORFs) and behavioral discrimination of similar odorants. We recorded from individual mitral cells in the OB in anesthetized rats in order to determine the degree of overlap in ORFs of individual mitral cells following exposure to odorant stimuli. Increasing the efficacy of the cholinergic neurotransmission in the OB by addition of the anticholinesterase drug neostigmine (20mM) sharpened the olfactory receptive field (ORF) responses of mitral cells. Furthermore, co-addition of either the nicotinic antagonist MLA (20mM) or muscarinic antagonist scopolamine (40mM) together with neostigmine (20mM) attenuated the neostigmine-dependent sharpening of ORF. These electrophysiological findings are predictive of accompanying behavioral experiments in which cholinergic modulation was manipulated by direct infusion of neostigmine, MLA and scopolamine into the OB during olfactory behavioral tasks. Increasing the efficacy of cholinergic action in the OB increased perceptual discrimination of odorants in these experiments, whereas blockade of nicotinic or muscarinic receptors decreased perceptual discrimination. These experiments show that behavioral discrimination is modulated in a manner predicted by the changes in mitral cell ORFs by cholinergic drugs. These results together present a first direct comparison between neural and perceptual effects of a bulbar neuromodulator.