Assessment of the decay rates of microbial source tracking molecular markers and faecal indicator bacteria from different sources
Assessment of the decay rates of microbial source tracking molecular markers and faecal indicator bacteria from different sources
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DOI：
10.1111/jam.14058
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发表时间：
2018-12-01
影响因子：
4
通讯作者：
Blanch, A. R.
中科院分区：
文献类型：
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作者：
Balleste, E.;Garcia-Aljaro, C.;Blanch, A. R.
目的评价T-90并比较不同粪便指示菌（FIB）和分子微生物源追踪（MST）标记物在夏季和冬季的人源和动物源的衰减。方法和结果结论使用围隔同时评估了大肠杆菌和肠球菌的持久性以及针对宿主特异性双歧杆菌和拟杆菌目物种（BifHM、BifCW、BifPL、HF 183/BFD、Rum 2Bac和Pig 2Bac）的几种MST分子标记。将装有来自不同来源的稀释废水的透析袋保存在室外水箱中，并定期监测以评估灭活率。E.大肠杆菌培养法夏季1个中心点52 ~ 5个中心点69天，冬季2个中心点06 ~ 6个中心点19天，而qPCR夏季2个中心点29-4个中心点23天，冬季4个中心点17-8个中心点09天。肠球菌的T-90值夏季为1中心点15至3中心点10天，冬季为3中心点01至5中心点46天。两种标记物的粪便来源之间观察到显著差异。对于MST标记物，在夏季获得了类似的T-90值（1个中心点05-1个中心点91天），而在冬季观察到更高的变异性（2个中心点90-6个中心点12天）。观察到不同来源的FIB的衰减速率不同，尤其是E。反刍动物样品中的大肠杆菌。在冬季不同的MST标记的T-90值之间观察到较高的变异性，而类似的T-90值在夏季检测到突出在这个季节的环境参数的影响较强。研究的意义和影响当这些速率被用来模拟水中的粪便污染时，在来自不同粪便来源的细菌中观察到的不同灭活速率具有影响。使用不同来源的FIBT 90对于开发可靠的预测模型至关重要。由于E.如果已经观察到关于污染源的大肠杆菌，则污染源必须被考虑用于建模方法。
Aims Evaluate the T-90 and compare the decay of different faecal indicator bacteria (FIB) and molecular microbial source tracking (MST) markers of human and animal sources during summer and winter. Methods and Results Conclusions The persistence of Escherichia coli and enterococci and several MST molecular markers targeting host-specific Bifidobacterium and Bacteroidales species (BifHM, BifCW, BifPL, HF183/BFD, Rum2Bac and Pig2Bac) was assessed at the same time using mesocosms. Dialysis bags filled with diluted wastewater from different sources were kept in an outdoor water tank and monitored regularly to assess the inactivation rates. The T-90 values of E. coli by culture methods ranged from 1 center dot 52 to 5 center dot 69 days in summer and 2 center dot 06 to 6 center dot 19 days in winter, whereas with qPCR 2 center dot 29-4 center dot 23 days in summer and 4 center dot 17-8 center dot 09 days in winter. T-90 values for enterocci ranged from 1 center dot 15 to 3 center dot 10 days in summer and from 3 center dot 01 to 5 center dot 46 days in winter. Significant differences were observed between faecal sources for both markers. For the MST makers similar T-90 values were obtained in summer (1 center dot 05-1 center dot 91 days), whereas higher variability was observed in winter (2 center dot 90-6 center dot 12 days). Different decay rates were observed for the FIB from the different sources, especially for E. coli in ruminant samples. A higher variability among T-90 values of the different MST markers in winter was observed, whereas similar T-90 values were detected in summer highlighting the stronger effect of environmental parameters during this season. Significance and Impact of the Study The diverse inactivation rates observed in bacteria from different faecal sources have implications when these rates are used to model faecal pollution in water. The use of FIBT90 of different sources is essential to develop reliable predictive models. Since different inactivation of E. coli regarding the source of pollution has been observed, the source of the pollution has to be considered for modelling approaches.