Exo utero electroporation of the mouse embryo
Exo utero electroporation of the mouse embryo
复制标题
小鼠胚胎的宫外电穿孔
DOI：
10.1007/978-1-4939-2459-2_2
复制
发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Tetsuichiro Saito
中科院分区：
文献类型：
--
作者：
Masakazu Hashimoto;Tatsuya Sato;Yuko Muroyama;Lisa Fujimura;Masahiko Hatano;Tetsuichiro Saito;Tetsuichiro Saito;Tetsuichiro Saito
通过子宫内电穿孔快速有效地转染核酸已经彻底改变了哺乳动物大脑的基因分析。然而，将核酸显微注射到目标部位并不容易，因为通过厚厚的子宫壁无法清晰地看到年轻的胚胎。为了克服这一困难，我们开发了体外电穿孔法。剖开孕鼠的子宫壁，露出含有胚胎的卵黄囊，可以清晰地看到胚胎，并易于操作进行电穿孔。电穿孔胚胎在母亲体内正常生长。体外电穿孔尤其适用于将其转染到发育中的脊髓和小脑以及将其局部转染到目标部位。
Quick and efficient transfection of nucleic acids by in utero electroporation has revolutionized gene analyses in the mammalian brain. Microinjection of nucleic acids into a targeted site, however, is not easy, because young embryos are not clearly visible through the thick uterine wall. To circumvent this difficulty, we developed the exo utero electroporation method. Incision of the uterine wall of a pregnant mouse exposes the yolk sac containing an embryo, and the embryo can be clearly visualized and easily manipulated for electroporation. The electroporated embryos grow normally in their mother’s body. Exo utero electroporation is particularly useful for transfection into the developing spinal cord and cerebellum and for focal transfection into a targeted site.