Clostridium difficile genotyping based on slpA variable region in S-layer gene sequence:: an alternative to serotyping
Clostridium difficile genotyping based on slpA variable region in S-layer gene sequence:: an alternative to serotyping
复制标题
基于 S 层基因序列中 slpA 可变区的艰难梭菌基因分型：血清分型的替代方法
DOI：
10.1128/jcm.40.7.2452-2458.2002
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发表时间：
2002-07-01
影响因子：
9.4
通讯作者：
Collignon, A
中科院分区：
文献类型：
--
作者：
Karjalainen, T;Saumier, N;Collignon, A
最近对艰难梭菌细胞壁组分的研究揭示了由slpA基因编码的S层的存在。本研究的目的是确定slpA基因分型是否可以作为血清分型的替代方法。随机选择血清型参考菌株和各种临床分离株，通过PCR扩增slpA的可变区。扩增产物经酶切和DNA测序后进行分析。发现SlpA蛋白的可变区的序列在给定血清群内是严格相同的，但在血清群之间是不同的。这些初步结果表明，PCR-限制性片段长度多态性，结合DNA测序的slpA可变区，可以构成一种替代的分型方法，用于确定艰难梭菌血清型。
Recent investigations of Clostridium difficile cell wall components have revealed the presence of an S-layer encoded by the slpA gene. The aim of this study was to determine whether slpA genotyping can be used as an alternative to serotyping. The variable regions of slpA were amplified by PCR from serogroup reference strains and various clinical isolates chosen randomly. Amplified products were analyzed after restriction enzyme digestion and DNA sequencing. The sequences of the variable region of the SlpA protein were found to be strictly identical within a given serogroup but divergent between serogroups. These preliminary results suggest that PCR-restriction fragment length polymorphism, in conjunction with DNA sequencing of the slpA variable region, could constitute an alternative typing method for determining C difficile serotypes.