DIFFERENTIAL RESPONSE OF MICROWAVE IRRADIATION-INDUCED DIFFERENTIATION AND DAMAGE IN PC12 MUTANT AND PC12 PARENTAL CELLS
DIFFERENTIAL RESPONSE OF MICROWAVE IRRADIATION-INDUCED DIFFERENTIATION AND DAMAGE IN PC12 MUTANT AND PC12 PARENTAL CELLS
复制标题
微波辐射诱导的 PC12 突变体和 PC12 亲本细胞分化和损伤的差异反应
DOI：
10.11418/jtca.36.31
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Kano Y
中科院分区：
文献类型：
--
作者：
Inoue S;Hiragami F;Kawamura K;Motoda H;Kawaura A;Kano Y
移动电话通信的日益使用引起了人们对微波辐射可能危害健康的担忧。我们研究了微波辐射对 PC12 突变细胞（PC12m3 细胞）的损伤和分化。 2.45 GHz (200 W) 微波辐射诱导的神经突生长频率比未辐射的对照细胞高约 10 倍。 PC12m3 细胞的微波处理导致 p38 MAPK 活性水平升高。同样，在 PC12m3 细胞中观察到 CREB 活性，但在 PC12 亲代细胞中未观察到 CREB 活性。 45℃热休克处理对PC12亲本细胞有较强的毒性作用，而PC12m3细胞对45℃和42℃温度均表现出比PC12亲本细胞更强的抵抗力。虽然200W微波照射对PC12m3细胞没有毒性作用，但微波照射导致PC12亲本细胞产生毒性。此外，100 W 的微波辐射并没有产生毒性作用，而是诱导 PC12m3 细胞增殖，尽管对 PC12 亲本细胞没有影响。这些发现表明，p38 MAPK 负责 PC12m3 细胞的存活和增殖，并可能通过微波照射下的 PC12m3 细胞中的 CREB 信号通路诱导神经突生长。
The increasing use of mobile phone communication has raised concerns about possible health hazard effects of microwave irradiation. We investigated damage and differentiation of PC12 mutant cells (PC12m3 cells) caused by microwave irradiation. The frequency of neurite outgrowth induced by 2.45 GHz (200 W) of microwave irradiation was approximately 10-fold greater than that in non-irradiated control cells. Microwave treatment of PC12m3 cells resulted in an increased level of p38 MAPK activity. Similarly, CREB activity was seen in PC12m3 cells but not in PC12 parental cells. Heat shock treatment at 45 C had a strong toxic effect on PC12 parental cells, whereas PC12m3 cells showed stronger resistance than PC12 parental cells to temperatures of both 45 C and 42 C. Although microwave irradiation at 200 W had no toxic effect on PC12m3 cells, microwave irradiation led to toxicity in PC12 parental cells. Moreover, microwave irradiation at 100 W, rather than having a toxic effect, induced proliferation of PC12m3 cells despite having no effect in PC12 parental cells. These findings indicate that p38 MAPK is responsible for the survival and proliferation of PC12m3 cells and might induce neurite outgrowth via a CREB signaling pathway in PC12m3 cells subjected to microwave irradiation.