A RAPID AND SENSITIVE MICROASSAY FOR DETERMINATION OF CHITINOLYTIC ACTIVITY
A RAPID AND SENSITIVE MICROASSAY FOR DETERMINATION OF CHITINOLYTIC ACTIVITY
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DOI：
10.1016/0167-7012(92)90055-9
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发表时间：
1992-01-01
影响因子：
2.2
通讯作者：
GOODAY, GW
中科院分区：
文献类型：
--
作者：
MCCREATH, KJ;GOODAY, GW
建立了一种在微尺度上快速、灵敏地测定几丁质水解活性的方法。该试验基于使用n -乙酰氨基葡萄糖寡糖的荧光性4-甲基伞草酮苷，以表征与几丁质降解微生物相关的酶，即外几丁质酶和内几丁质酶以及n -乙酰氨基葡萄糖酶。该分析的灵敏度允许进行详细的动力学研究。举例说明其用于从河口细菌分离物中分解几丁质。
A rapid and sensitive assay for chitinolytic activity has been developed on a micro-scale. This assay is based on the use of fluorogenic 4-methylumbelliferone glycosides of N-acetylglucosamine oligosaccharides in order to characterise the enzymes associated with chitinolytic microorganisms, i.e. exo- and endochitinases and N-acetylglucosamimidase. The sensitivity of the assay allows detailed kinetic studies to be performed. Examples of its uses are given for chitinolytic activities from an estuarine bacterial isolate.