Effectiveness of Acid-electrolyzed Functional Water for Mouth Wash: An In Vitro Study
Effectiveness of Acid-electrolyzed Functional Water for Mouth Wash: An In Vitro Study
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DOI：
10.21873/invivo.12947
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发表时间：
2022
期刊：
影响因子：
2.3
通讯作者：
Daichi Oikawa;Kensuke Nishio;M. Tamura;Mai Fukasawa;Takamasa Yoshida;Shinji Okada;Tomoka Ito;Mariko Tsunoda;M. Asano;T. Iinuma
中科院分区：
文献类型：
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作者：
Daichi Oikawa;Kensuke Nishio;M. Tamura;Mai Fukasawa;Takamasa Yoshida;Shinji Okada;Tomoka Ito;Mariko Tsunoda;M. Asano;T. Iinuma
摘要背景/目的：酸性功能水(FW)是一种有效的杀菌剂，用FW漱口可能是一种有效的口腔护理方法。我们通过一项体外研究，研究了FW作为漱口剂的可能性。材料和方法：以菌落形成单位(CFU)为指标评价FW对不同细菌(金黄色葡萄球菌、肺炎链球菌、铜绿假单胞菌、白色念珠菌)的杀菌效果。实验使用PBS、Listerine和ConCool F(未稀释，并指明了最佳浓度)。为探讨FW的杀菌机理，测定了金黄色葡萄球菌体内氧化作用的指示物超氧化物歧化酶(SOD)的活性。FW用纯净水稀释至10%、30%、50%和70%。测定每种浓度下的CFU数。使用HSC-3和HeLa细胞进行XTT检测，以检测FW处理后细胞的活力。用PBS、Listerine和未稀释的ConCool F进行了相同的实验。结果：FW处理的细菌没有形成菌落。SOD活性在FW浓度为50%时达到最大值，是对照的两倍多。观察到50%治疗后CFU数量显著减少。由于超氧化物歧化酶活性峰值与杀菌效果的起始浓度重合，FW的杀菌作用可能与其氧化作用有关。用FW处理的细菌的存活率与其他洗口液相同。结论：口服液可作为含漱剂应用于临床。
Abstract Background/Aim: Acid-electrolyzed functional water (FW) is an efficient bactericide and gargling with FW might be an effective method of oral care. We investigated the possible use of FW as a mouth wash by an in vitro study. Materials and Methods: The bactericidal effect of FW against different species of bacteria (Staphylococcus aureus, Streptococcus pneumonia, Pseudomonas aeruginosa, and Candida albicans) was evaluated using the numbers of colony-forming units (CFU). The experiment was conducted using PBS, LISTERINE, and ConCool F (undiluted, and the optimal concentration indicated). To investigate the bactericidal mechanism of FW, the activity of superoxide dismutase (SOD), an indicator of oxidative action, was measured in S. aureus. FW was diluted with purified water to concentrations of 10, 30, 50, and 70%. The numbers of CFU were measured for each concentration. XTT assays were performed using HSC-3 and HeLa cells, to examine the viability of the cells following treatment with FW. The same experiment was conducted with PBS, LISTERINE, and undiluted ConCool F. Results: No bacteria treated with FW formed colonies. SOD activity peaked at a 50% concentration of FW and was more than twice that of the control. A significant decrease in the number of CFU was observed following 50% treatment. Since the peaks of the SOD activity and the starting concentrations of the bactericidal effects coincided, the bactericidal effect of FW might be related to its oxidative effects. Bacteria treated with FW had the same survival rate as the other mouth washes. Conclusion: FW might be clinically applicable as a mouth wash.