Principal component analysis of direct matrix-assisted laser desorption/ionization mass spectrometric data related to metabolites of fatty liver.
Principal component analysis of direct matrix-assisted laser desorption/ionization mass spectrometric data related to metabolites of fatty liver.
复制标题
与脂肪肝代谢物相关的直接基质辅助激光解吸/电离质谱数据的主成分分析。
DOI：
10.5650/jos.58.267
复制
发表时间：
2009
影响因子：
1.5
通讯作者：
M. Setou
中科院分区：
文献类型：
--
作者：
N. Zaima;Y. Matsuyama;M. Setou
非酒精性脂肪性肝炎（NASH）是一种常见的肝脏疾病。NASH的特征在于脂肪肝，沿着炎症。大多数NASH患者并不知道自己的病情，尽管NASH可能导致肝硬化。已经测试了几种方法来阐明NASH的病理学。然而，NASH的发病机制尚未明确。在这项研究中，一个监督的多变量分析（主成分分析）的方法，使用直接基质辅助激光解吸/电离质谱（dMALDI-MS）的代谢物饥饿诱导的脂肪肝组织切片的分析。这种方法不需要复杂的预处理。我们研究了脂肪肝中代谢物的特征动力学。该方法可用于脂肪肝活检标本的分析。
Non-alcoholic steatohepatitis (NASH) is a common liver disease. NASH is characterized by fatty liver, along with inflammation. Most people with NASH are not aware of their condition, even though NASH can lead to hepatic cirrhosis. Several approaches have been tested to clarify the pathology of NASH. However, the mechanism of onset of NASH was not well-defined. In this study, a supervised multivariate analysis (principal component analysis) approach using direct matrix-assisted laser desorption/ionization mass spectrometry (dMALDI-MS) was applied to the analysis of metabolites in starvation-induced fatty liver tissue sections. This approach does not require complex pretreatments. We investigated the characteristic dynamics of metabolites in fatty liver. This approach can be applied to the analysis of human biopsy specimens of fatty liver in future studies.