High-performance liquid chromatographic determination of imidazole dipeptides, histidine, 1-methylhistidine and 3-methylhistidine in equine and camel muscle and individual muscle fibres
High-performance liquid chromatographic determination of imidazole dipeptides, histidine, 1-methylhistidine and 3-methylhistidine in equine and camel muscle and individual muscle fibres
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DOI：
10.1016/s0378-4347(97)88054-1
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发表时间：
1997-01-10
期刊：
影响因子：
--
通讯作者：
Harris, RC
中科院分区：
文献类型：
--
作者：
Dunnett, M;Harris, RC
提出的固相萃取(Isolte PRS柱)和反相梯度高效液相色谱相结合的方法，提供了灵敏、重现性和选择性地定量测定马和骆驼肌肉和单个肌肉纤维中的肌氨酸、安丝氨酸、香豆碱、高肌氨酸、组氨酸、1-甲基组氨酸和3-甲基组氨酸。回收率为91-115%。最低检出限为0.005~0.010 mmolkg(-1)干肌肉。这些化合物是从其他生理氨基酸和小肽中分离出来的，并在55min的单层析运行中得到拆分。这些化合物在马的心肌、横隔膜、骨骼肌、骆驼肌和两个物种的单个肌肉纤维中的浓度是第一次被公布。
The combined solid-phase extraction (Isolute PRS columns) and reversed-phase gradient HPLC method presented provides a sensitive, reproducible and selective quantification of carnosine, anserine, balenine, homocarnosine, histidine, 1-methylhistidine and 3-methylhistidine in equine and camel muscle and individual muscle fibres. Recoveries were 91-115%. Lower limits of detection were 0.005-0.010 mmol kg(-1) dry muscle. The compounds were isolated from other physiological amino acids and small peptides and resolved within a single chromatographic run of 55 min. Concentrations of these compounds in equine myocardium, diaphragm, skeletal muscle, camel muscle and individual muscle fibres of both species are presented for the first time.