A pilot study using metagenomic sequencing of the sputum microbiome suggests potential bacterial biomarkers for lung cancer.
A pilot study using metagenomic sequencing of the sputum microbiome suggests potential bacterial biomarkers for lung cancer.
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DOI：
10.1371/journal.pone.0177062
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Mur LAJ
中科院分区：
文献类型：
--
作者：
Cameron SJS;Lewis KE;Huws SA;Hegarty MJ;Lewis PD;Pachebat JA;Mur LAJ
肺癌 (LC) 是全球最常见的癌症，每年导致超过 130 万人死亡。目前，与其他癌症相比，LC 的五年生存率较低，因此需要新的方法来筛查和诊断恶性肿瘤，以改善患者的预后。在这里，我们报告了一项试点规模的研究，以评估痰微生物组作为肺癌状态和分期的非侵入性细菌生物标志物来源的潜力。从 10 名可能患有 LC 的患者中收集了自发痰样本，其中 4 名最终诊断为 LC (LC+)，6 名一年后未诊断出 LC (LC-)。在所有样品中发现的七种细菌中，草绿色链球菌在 LC+ 样品中显着较高。仅在 LC- 样品中发现了另外 7 种细菌，仅在 LC+ 样品中发现了 16 种细菌。在 LC+ 和 LC-病例之间的显着倍数变化方面还发现了其他分类学差异，其中 5 个物种的 LC+ 丰度显着较高，其中 Granulicatella adiacens 显示出最高水平的丰度变化。功能差异通过显着的倍数变化显而易见，包括多胺代谢和铁铁载体受体。 G. adiacens 丰度与其他六种细菌（即肠球菌属 sp.）相关。 130、中间链球菌、大肠杆菌、草绿色链球菌、君氏不动杆菌和链球菌。 6，仅在 LC+ 样品中，这也可能与 LC 阶段有关。自发痰似乎是细菌生物标志物的可行来源，可用作 LC 状态和分期的生物标志物。
Lung cancer (LC) is the most prevalent cancer worldwide, and responsible for over 1.3 million deaths each year. Currently, LC has a low five year survival rates relative to other cancers, and thus, novel methods to screen for and diagnose malignancies are necessary to improve patient outcomes. Here, we report on a pilot-sized study to evaluate the potential of the sputum microbiome as a source of non-invasive bacterial biomarkers for lung cancer status and stage. Spontaneous sputum samples were collected from ten patients referred with possible LC, of which four were eventually diagnosed with LC (LC+), and six had no LC after one year (LC-). Of the seven bacterial species found in all samples, Streptococcus viridans was significantly higher in LC+ samples. Seven further bacterial species were found only in LC-, and 16 were found only in samples from LC+. Additional taxonomic differences were identified in regards to significant fold changes between LC+ and LC-cases, with five species having significantly higher abundances in LC+, with Granulicatella adiacens showing the highest level of abundance change. Functional differences, evident through significant fold changes, included polyamine metabolism and iron siderophore receptors. G. adiacens abundance was correlated with six other bacterial species, namely Enterococcus sp. 130, Streptococcus intermedius, Escherichia coli, S. viridans, Acinetobacter junii, and Streptococcus sp. 6, in LC+ samples only, which could also be related to LC stage. Spontaneous sputum appears to be a viable source of bacterial biomarkers which may have utility as biomarkers for LC status and stage.