Microfabrication of homogenous, asymmetric cell-laden hydrogel capsules.
Microfabrication of homogenous, asymmetric cell-laden hydrogel capsules.
复制标题
同质，非对称细胞水凝胶胶囊的微结构。
DOI：
10.1016/j.biomaterials.2009.09.012
复制
发表时间：
2009-12
期刊：
影响因子：
14
通讯作者：
Anderson DG
中科院分区：
文献类型：
--
作者：
Dang TT;Xu Q;Bratlie KM;O'Sullivan ES;Chen XY;Langer R;Anderson DG
细胞包封作为一种为移植的内分泌细胞提供免疫保护的技术已被广泛研究。在这里，我们开发了新的制造方法，允许快速，均匀的微囊化胰岛素分泌细胞与不同的微观几何形状和不对称的修改表面。微成型系统开发使用聚丙烯网，并确定与有效封装的材料/表面性能。使用这些方法包封的细胞保持所需的活力，并保持其以葡萄糖响应性方式增殖和分泌胰岛素的能力。这种新的细胞包封方法使得能够实现用于产生载有细胞的微胶囊的廉价且方便的方法的实用路线，而不需要任何专门的设备或微制造工艺。
Cell encapsulation has been broadly investigated as a technology to provide immunoprotection for transplanted endocrine cells. Here we develop new fabrication methods that allow for rapid, homogenous microencapsulation of insulin-secreting cells with varying microscale geometries and asymmetrically modified surfaces. Micromolding systems were developed using polypropylene mesh, and the material/surface properties associated with efficient encapsulation were identified. Cells encapsulated using these methods maintain desirable viability and preserve their ability to proliferate and secrete insulin in a glucose-responsive manner. This new cell encapsulation approach enables a practical route to an inexpensive and convenient process for the generation of cell-laden microcapsules without requiring any specialized equipment or microfabrication process.