Double-stranded RNA specifically disrupts gene expression during planarian regeneration
Double-stranded RNA specifically disrupts gene expression during planarian regeneration
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DOI：
10.1073/pnas.96.9.5049
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发表时间：
1999-04-27
影响因子：
11.1
通讯作者：
Newmark, PA
中科院分区：
文献类型：
--
作者：
Alvarado, AS;Newmark, PA
后生动物的再生是生物学中最不为人所知的基本问题之一。在分子水平上理解这种现象缺乏进展，原因是用于发育研究的遗传生物的再生能力较差，以及通常研究的脊椎动物模型（有尾目两栖动物）的分子和遗传操作遇到的困难。在这里，我们证明引入双链 RNA 可以选择性地消除涡虫中的基因功能，涡虫是一种经典的再生模型。消除涡虫等再生生物体中基因功能的能力克服了先前的实验限制，并将动物再生研究开启到前所未有的分子细节水平。
Metazoan regeneration is one of the least understood fundamental problems of biology. The lack of progress in understanding this phenomenon at the molecular level has been due to the poor regenerative abilities of the genetic organisms used for developmental studies, as well as the difficulties encountered with molecular and genetic manipulations of the commonly studied vertebrate models (the urodele amphibians). Here, we demonstrate that introduction of double-stranded RNA selectively abrogates gene function in planarians, a classic model of regeneration. The ability to eliminate gene function in a regenerating organism such as the planarian overcomes previous experimental limitations and opens the study of animal regeneration to unprecedented levels of molecular detail.