The synovitis of "non-inflammatory'' orthopaedic arthropathies: a quantitative histological and immunohistochemical analysis
The synovitis of "non-inflammatory'' orthopaedic arthropathies: a quantitative histological and immunohistochemical analysis
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DOI：
10.1136/ard.2008.087775
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发表时间：
2008-08-01
影响因子：
27.4
通讯作者：
Schumacher, H. R.
中科院分区：
文献类型：
--
作者：
Pessler, F.;Dai, L.;Schumacher, H. R.
目的：量化骨科“非炎症性”关节病（Orth. A）.方法：股骨骨折、股骨缺血性坏死、滑膜皱襞综合征和半月板和/或韧带损伤患者的滑膜（n=23）;类风湿性关节炎（n=28）;骨关节炎OA组25例;通过光学显微镜、组织学滑膜炎评分、CD 3、CD 20、CD 38、CD 68、Ki-67和von Willebrand因子的免疫染色，结果：① ①正常对照组与正常对照组相比，差异有统计学意义（P < 0. 05）;组织学在正常和明显发炎之间变化。主要异常为轻度内膜增生、散在炎细胞和血管周围小浸润。滑膜炎评分为Orth。A为“轻度滑膜炎”。炎性细胞多见，100%为CD 68+细胞。A组标本CD 3 + 91%，CD 38 + 70%，CD 20 + 39%。奥斯与正常对照组相比，A组内膜厚度增加36%（p=0.04），血管密度增加40%（p=0.009），CD 38+细胞密度增加51.3倍（p=0.02）;内膜下Ki-67+细胞减少60%（p=0.003），CD 68+内膜细胞减少42%（p=0.003），
Objective: To quantify inflammatory changes in synovial membranes from orthopaedic "non-inflammatory'' arthropathies (Orth. A).Methods: Synovial membranes from patients with femur fracture, avascular necrosis of the femur, plica syndrome, and meniscus and/or ligament injury (n=23); rheumatoid arthritis (n=28); osteoarthritis (OA; n=25); and from normal controls (n=10) were assessed by light microscopy, a histological synovitis score, immunostaining for CD3, CD20, CD38, CD68, Ki-67 and von Willebrand factor, and with an immunohistochemical inflammation score.Results: Orth. A histology varied between normal and markedly inflamed. Predominant abnormalities were mild lining hyperplasia, scattered inflammatory cells and small perivascular infiltrates. The synovitis score classified Orth. A as "mild synovitis''. Inflammatory cells occurred frequently: CD68+ cells in 100% of Orth. A specimens; CD3+, 91%; CD38+, 70%; and CD20+, 39%. Orth. A had 36% greater lining thickness (p=0.04), 40% higher vascular density (p=0.009) and 51.3-fold higher CD38+ cell density (p=0.02) than normal controls; and 60% fewer subintimal Ki-67+ cells (p=0.003), 42% fewer CD68+ lining cells (p