ADSORPTION OF BACTERIOPHAGE PHIX174 TO ITS HOST
ADSORPTION OF BACTERIOPHAGE PHIX174 TO ITS HOST
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DOI：
10.1016/s0006-3495(62)86866-0
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发表时间：
1962-01-01
影响因子：
3.4
通讯作者：
KAESBERG, P
中科院分区：
文献类型：
--
作者：
FUJIMURA, R;KAESBERG, P
研究了纯化后的[phi]X174在大肠杆菌C和大肠杆菌C细胞壁上的吸附作用。通过测定未吸附的斑块形成物来测定吸附。不可逆和可逆吸附量取决于pH值和二价离子浓度。最大不可逆吸附发生在0.1[图像]CaCl2在36CC。在最适合不可逆吸附的pH和CaCl2浓度条件下，没有检测到可逆吸附。在这些最佳条件下，扩散不是速率限制因素，并且遇到效率显得很低。速率常数为1.0 × 10- 10ml /秒。不可逆地吸附在活细胞上的噬菌体引起感染，吸附在分离的细胞壁上显然引起DNA的释放。在细胞壁的黏液复合物部分有一个特殊的[phi]X174受体位点。
The adsorption of purified [phi]X174 to E. coli C and to E. coli C cell walls was investigated. Adsorption was measured by assaying for unadsorbed plaque formers. The amount of irreversible and reversible adsorption depends upon pH and divalent ion concentration. Maximum irreversible adsorption occurs in 0.1 [image] CaCl2 at 36CC. There is no detectable reversible adsorption at conditions of pH and CaCl2 concentration optimum for irreversible adsorption. Under these optimum conditions, diffusion is not the rate-limiting factor, and the encounter efficiency appears to be low. The rate constant is 1.0 X 10- 10 ml/sec. Phages adsorbed irreversibly to live cells cause infection and to the isolated cell walls apparently cause release of DNA. There is a specific [phi]X174 receptor site on the mucocomplex portion of the cell wall.