Nucleotide sequence of a Euglena gracilis chloroplast genome region coding for the elongation factor Tu; evidence for a spliced mRNA.
Nucleotide sequence of a Euglena gracilis chloroplast genome region coding for the elongation factor Tu; evidence for a spliced mRNA.
复制标题
编码延伸因子Tu的细小眼虫叶绿体基因组区域的核苷酸序列；
DOI：
10.1093/nar/11.17.5877
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发表时间：
1983
影响因子：
14.9
通讯作者：
E. Stutz
中科院分区：
文献类型：
--
作者：
P. Montandon;E. Stutz
通过S1核酸酶分析和DNA测序，我们鉴定了纤细眼虫叶绿体DNA片段Eco-N + Q的1.95 kb转录产物，并表明它是三个剪接事件的产物。外显子1（0.45 kb）、外显子2（0.74 kb）和外显子3（0.53 kb）的175个核苷酸编码叶绿体延伸因子蛋白（EF-Tu）。外显子3和外显子4的剩余部分（0.23 kb）具有未鉴定的开放阅读框。叶绿体EF-Tu蛋白由408个氨基酸组成，与E. coli EF-Tu蛋白。氨酰-tRNA结合的活性位点是高度保守的，而GTP/GDP结合的活性位点缺乏E. coli EF-Tu蛋白。分隔外显子1、2和3的两个内含子分别为103和110个核苷酸长。第三个内含子的大小尚未确定。不遵循真核生物mRNA的剪接规则。
We characterize a 1.95 kb transcription product of the Euglena gracilis chloroplast DNA fragment Eco-N + Q by S1 nuclease analysis and DNA sequencing and show that it is the product of three splicing events. Exon 1 (0.45 kb), exon 2 (0.74 kb) and 175 nucleotides of exon 3 (0.53 kb) code for the chloroplast elongation factor protein (EF-Tu). The remaining part of exon 3 and exon 4 (0.23 kb) have unidentified open reading frames. The chloroplast EF-Tu protein has 408 aminoacids and is to 70% homologous with the E. coli EF-Tu protein. The active site for aminoacyl-tRNA binding is highly conserved, while the active site for GTP/GDP binding lacks the cysteine present in the E. coli EF-Tu protein. The two introns separating exons 1, 2 and 3 are, respectively, 103 and 110 nucleotides long. The size of the third intron is not yet determined. The splicing rules for eukaryote mRNA are not followed.