Transient Increase of Cytokines in the Acute Ischemic Tissue is Beneficial to Cell-Based Therapeutic Angiogenesis
Transient Increase of Cytokines in the Acute Ischemic Tissue is Beneficial to Cell-Based Therapeutic Angiogenesis
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DOI：
10.1253/circj.cj-08-0392
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发表时间：
2008-12-01
影响因子：
3.3
通讯作者：
Hamano, Kimikazu
中科院分区：
文献类型：
--
作者：
Qin, Shu-Lan;Li, Tao-Sheng;Hamano, Kimikazu
背景骨髓细胞(BMC)移植是治疗还是缺血性疾病。众所周知，许多炎性细胞因子在缺血组织中释放，特别是在急性期，因此本研究旨在探讨急性缺血组织中细胞因子的一过性增加是否影响基于细胞的治疗性血管生成。方法与结果建立C57BL/6小鼠缺血后24 h(急性)和2周(慢性)的肢体缺血模型。用从急、慢性缺血肌肉中提取的总组织蛋白培养骨髓细胞。急性缺血肢体提取的总组织蛋白浓度为1 mg/ml时，骨髓单个核细胞的存活、黏附和迁移能力明显好于慢性缺血肢体(P
Background Implantation of bone marrow cells (BMCs) is it treatment or ischemic disease. It is well known that many inflammatory cytokines are released in ischemic tissue, especially in the acute phase, so in the present Study it was investigated if the transient increase of cytokines in the acute ischemic tissue influences cell-based therapeutic angiogenesis.Methods and Results Ischemic limb models were created in C57BL/6 mice as 24 h (acute) or 2 weeks (chronic) after ischemia. BMCs were cultured With total tissue protein, which was extracted from the acute and chronic ischemic Muscles. The survival, adhesion, and migration of BMCs were significantly better after culture with 1 mg/ml total tissue protein extracted from the acute ischemic limbs than from the chronic ischemic limbs (p