Expression and Functional Analysis of the ZCN1(ZmTFL1) Gene, a TERMINAL FLOWER 1 Homologue that Regulates the Vegetative to Reproductive Transition in Maize
Expression and Functional Analysis of the ZCN1(ZmTFL1) Gene, a TERMINAL FLOWER 1 Homologue that Regulates the Vegetative to Reproductive Transition in Maize
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DOI：
10.1007/s11105-011-0317-2
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发表时间：
2011-05
影响因子：
2.1
通讯作者：
Lili Chang;Liancheng Wu;Yanhui Chen;L. Ku;Shuang Yang;Shaofang Zhang;Xintao Wang;Xiaomin Wei
中科院分区：
文献类型：
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作者：
Lili Chang;Liancheng Wu;Yanhui Chen;L. Ku;Shuang Yang;Shaofang Zhang;Xintao Wang;Xiaomin Wei
末端花1号(TFL1)同源基因在调节分生组织的开花时间和/或维持分生组织的开花中起着重要作用。本研究从热带自交系CML288和温带自交系黄早4号中克隆了玉米TFL1正交基因ZmTFL1(ZCN1)，并对ZmTFL1(ZCN1)在不同花发育时期的功能进行了研究。时空表达模式表明，ZCN1主要定位于发育成花的顶端分生组织，在叶片中仅有少量表达。为了进一步了解ZCN1在玉米花发育中的作用，利用激光扫描共聚焦显微镜对玉米花发育过程中的顶端形态进行了研究。并测定了ZCN1、ZCN8、DLF1和ZAP1基因的相对表达水平。ZCN1的过度表达部分补充了拟南芥突变体tfl1-14的晚花表型。此外，转基因拟南芥分裂株的花序不定，枝长增加，叶片上的毛状体数量增加。此外，在35S：：ZCN1转基因拟南芥裂解体中，AP1的表达水平显著下调。这些结果表明，ZCN1及其在拟南芥中的同源基因TFL1参与了玉米花器转换的调控。
TERMINAL FLOWER 1(TFL1) homologs play critical roles in regulating flowering time and/or maintaining flowering of meristems. In this study, the gene of maizeTFL1orthologZmTFL1(ZCN1) was cloned from both the tropical inbred line CML288 and temperate inbred line Huangzao 4, and the function ofZmTFL1(ZCN1) was determined during different periods of floral development. Spatial and temporal expression patterns revealed thatZCN1was predominantly localized in shoot apical meristems that develop into flowers, and only at low levels in leaves. To further identify the role ofZCN1in floral development of maize, the morphology of shoot apices in maize during floral development was investigated using laser scanning confocal microscopy. Moreover, the relative levels of expression ofZCN1,ZCN8,DLF1, andZAP1genes were determined. Over-expression ofZCN1partially complemented the late flowering phenotype in the tfl1-14Arabidopsismutant. Moreover, transgenicArabidopsisplants exhibited indeterminate inflorescence with increased shoot length and higher numbers of trichomes on leaves. In addition, expression levels ofAP1were significantly down-regulated in35S::ZCN1transgenicArabidopsisplants. These results indicated thatZCN1as well as its homologTFL1inArabidopsisare involved in the regulation of floral transition in maize.