Retinoblastoma protein prevents staurosporine-induced cell death in a retinoblastoma-defective human glioma cell line
Retinoblastoma protein prevents staurosporine-induced cell death in a retinoblastoma-defective human glioma cell line
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DOI：
10.1159/000101048
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发表时间：
2007-01-01
期刊：
影响因子：
5
通讯作者：
Kurisu, Kaoru
中科院分区：
文献类型：
--
作者：
Yamasaki, Fumiyuki;Kajiwara, Yoshinori;Kurisu, Kaoru
目的：研究腺病毒介导的基因转染法诱导星形胶质瘤细胞死亡和细胞周期停滞的机制，以及星形孢菌素诱导的视网膜母细胞瘤(RB)的功能和遗传不稳定性。方法：应用表达Rb、p16和p21基因的腺病毒载体，观察星形孢子素对人脑胶质瘤细胞周期调控、细胞死亡和核异常的影响。结果：与完整表达Rb的细胞株相比，Rb缺陷的SF-539细胞株对星形孢子素耐药。SF-539胶质瘤细胞暴露于星形孢子素后出现多核，然后死亡。在转导Rb基因的SF-539细胞中，防止了多核形成，从而降低了这些细胞的死亡率。结论：这些结果表明，增强的Rb表达保护细胞免受星状孢子素诱导的基因组不稳定，而不考虑其上游分子效应。版权所有(C)2007 S.Karger AG，巴塞尔。
Objective: To investigate the mechanism of staurosporine-induced glioma cell death and cell cycle arrest using adenovirus-mediated gene transfection, as well as the function of retinoblastoma (Rb) and genetic instability induced by staurosporine. Methods: Cell cycle regulation, cell death and nuclear abnormalities induced by staurosporine were examined using an adenovirus vector expressing Rb, p16 or p21 genes in human glioma cell lines. Results: The Rb-defective SF-539 cell line was resistant to staurosporine compared with cell lines expressing intact Rb. SF-539 glioma cells exposed to staurosporine became multinucleated and then died. Multinucleation was prevented in SF-539 cells transfected with the Rb gene, thus decreasing the death rate of these cells. Conclusions: These results imply that enforced Rb expression protects cells from genomic instability induced by staurosporine regardless of its upstream molecular effects. Copyright (c) 2007 S. Karger AG, Basel.