Two-dimensional microarray of HepG2 spheroids using collagen/polyethylene glycol micropatterned chip
Two-dimensional microarray of HepG2 spheroids using collagen/polyethylene glycol micropatterned chip
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DOI：
10.1007/s10856-007-3305-1
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发表时间：
2008-05-01
影响因子：
3.7
通讯作者：
Nakazawa, K.
中科院分区：
文献类型：
--
作者：
Tamura, T.;Sakai, Y.;Nakazawa, K.
利用微接触印刷技术，开发了一种新的细胞芯片技术，即HepG 2球状体的二维微阵列。该芯片由玻璃板（24 x 24 mm）中心100 mm（2）区域内呈三角形排列的几个胶原蛋白斑点组成，该区域作为细胞粘附区域;不包括用聚乙二醇（PEG）修饰的胶原蛋白斑点的区域作为非粘附区域。接种到芯片上的HepG 2细胞由于细胞增殖，逐渐在每个胶原斑点上形成表面光滑、圆形度高的球状体，培养10天后球状体直径保持不变。这种HepG 2球状体的二维微阵列配置可以维持至少2周。球体直径与芯片上胶原斑点之间的间距成正比。这表明我们可以人为地控制球体的直径。此外，HepG 2球状体的白蛋白分泌活性随着球状体直径的增加而增加。该芯片技术可用作开发二维球状体微阵列的细胞平台。
A new cell chip technology in the form of a two-dimensional microarray of HepG2 spheroids was developed by using the microcontact printing technique. The chip consisted of several collagen spots in a triangular arrangement within a 100-mm(2) area at the center of a glass plate (24 x 24 mm), which served as the cell adhesion area; the region excluding the collagen spots that was modified with polyethylene glycol (PEG) served as the non-adhesion area. HepG2 cells inoculated onto the chip gradually formed spheroids with smooth surfaces and high circularity on each collagen spot due to cell proliferation; the spheroid diameters remained constant after 10 days of culture. Such a two-dimensional microarray configuration of HepG2 spheroids could be maintained for at least 2 weeks. The spheroid diameter was directly proportional to the pitch between the collagen spots on the chip. This indicates that we can factitiously control the spheroid diameter. In addition, albumin secretion activity of HepG2 spheroids increased with the increase of spheroid diameter. This chip technology may be applicable as a cellular platform for developing two-dimensional spheroid microarrays.