Human somatostatin I: sequence of the cDNA.
Human somatostatin I: sequence of the cDNA.
复制标题
人生长抑素 I：cDNA 序列。
DOI：
10.1073/pnas.79.15.4575
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发表时间：
1982
影响因子：
11.1
通讯作者：
Rutter,WJ
中科院分区：
文献类型：
--
作者：
Shen,LP;Pictet,RL;Rutter,WJ
从人胰腺生长抑素瘤中提取了RNA，并用于制备cDNA文库。经过预筛选，含有生长抑素I序列的克隆通过与生长抑素I克隆的cDNA探针杂交进行鉴定。从其中两个克隆的核苷酸序列中，我们推导出一个基本上全长的mRNA序列，包括前生长抑素编码区、5‘非翻译区的105个核苷酸和完整的150个核苷酸的3’非翻译区。该编码区预测了一个由116个氨基酸组成的前体蛋白(MR，12.727)，其COOH端含有生长抑素-14和-28。预测的人生长抑素-28的氨基酸序列与从猪和绵羊中分离的生长抑素-28的氨基酸序列相同。对人和鳗鱼前生长抑素I的氨基酸序列的比较表明，编码生长抑素-14的COOH末端及其邻近的6个氨基酸高度保守，而分子的其余部分，包括信号肽区域，则更加分化。然而，在人类和鳗鱼蛋白的PRO区域发现的许多氨基酸差异是保守的变化。这表明前肽具有相似的二级结构，这反过来可能意味着分子的这一区域具有生物功能。
RNA has been isolated from a human pancreatic somatostatinoma and used to prepare a cDNA library. After prescreening, clones containing somatostatin I sequences were identified by hybridization with an anglerfish somatostatin I-cloned cDNA probe. From the nucleotide sequence of two of these clones, we have deduced an essentially full-length mRNA sequence, including the preprosomatostatin coding region, 105 nucleotides from the 5' untranslated region and the complete 150-nucleotide 3' untranslated region. The coding region predicts a 116-amino acid precursor protein (Mr, 12.727) that contains somatostatin-14 and -28 at its COOH terminus. The predicted amino acid sequence of human somatostatin-28 is identical to that of somatostatin-28 isolated from the porcine and ovine species. A comparison of the amino acid sequences of human and anglerfish preprosomatostatin I indicated that the COOH-terminal region encoding somatostatin-14 and the adjacent 6 amino acids are highly conserved, whereas the remainder of the molecule, including the signal peptide region, is more divergent. However, many of the amino acid differences found in the pro region of the human and anglerfish proteins are conservative changes. This suggests that the propeptides have a similar secondary structure, which in turn may imply a biological function for this region of the molecule.