Influence of the scattering phase function approximation on the optical properties of blood determined from the integrating sphere measurements.

Influence of the scattering phase function approximation on the optical properties of blood determined from the integrating sphere measurements.
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散射相函数近似对积分球测量确定的血液光学特性的影响。

DOI:
10.1117/1.429920
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发表时间:
1999
影响因子:
3.5
通讯作者:
H. Schwarzmaier
H. Schwarzmaier
中科院分区:
医学3区
文献类型:
--
作者:
A. Yaroslavsky;I. Yaroslavsky;Thomas Goldbach;H. Schwarzmaier

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我们研究了散射相函数近似的光学性质的人全血确定从积分球测量使用逆蒙特卡罗技术的影响。采用双积分球法测量全血样品(Hct=38%)的漫反射率Rd和总透射率Tt(λ=633 nm)。使用测角光度计测量高度稀释的血液样品(Hct=0.1%)的散射相函数。我们用Henyey-Greenstein(HGPF)、Gegenbauer核(GKPF)或Mie(MPF)相函数来近似实验确定的散射相函数,以预设反问题的各向异性因子μ ′。我们在逆蒙特卡罗程序中采用了HGPF、GKPF和MPF近似来导出吸收系数μa和散射系数μs。为了评估所获得的数据,我们计算了光学厚样品的散射光的角分布,并与测角光度测量结果进行了比较。本研究中提供的数据表明,所采用的散射相函数的近似可以对μs和μ '的导出值产生实质性影响,而μa和约化散射系数μs'对散射相函数的精确形式不太敏感。© 1999年摄影光学仪器工程师协会。
We investigated the impact of the scattering phase function approximation on the optical properties of whole human blood determined from integrating sphere measurements using an inverse Monte Carlo technique. The diffuse reflectance Rd and the total transmittance Tt(λ=633 nm) of whole blood samples (Hct=38%) were measured with double-integrating sphere equipment. The scattering phase functions of highly diluted blood samples (Hct=0.1%) were measured using a goniophotometer. We approximated the experimentally determined scattering phase functions with either Henyey-Greenstein (HGPF), Gegenbauer kernel (GKPF), or Mie (MPF) phase functions to preset the anisotropy factor μ¯ for the inverse problem. We have employed HGPF, GKPF, and MPF approximations in the inverse Monte Carlo procedure to derive the absorption coefficient μa and the scattering coefficient μs. To evaluate the obtained data, we calculated the angular distributions of scattered light for optically thick samples and compared the results with goniophotometric measurements. The data presented in this study demonstrate that the employed approximation of the scattering phase function can have a substantial impact on the derived values of μs and μ¯, while μa and the reduced scattering coefficient μs' are much less sensitive to the exact form of the scattering phase function. © 1999 Society of Photo-Optical Instrumentation Engineers.