Molecular analysis of early rice stamen development using organ-specific gene expression profiling
Molecular analysis of early rice stamen development using organ-specific gene expression profiling
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使用器官特异性基因表达谱对早期水稻雄蕊发育进行分子分析
DOI：
10.1007/s11103-006-0054-3
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发表时间：
2006-08-01
影响因子：
5.1
通讯作者：
Bai, Shu-Nong
中科院分区：
文献类型：
--
作者：
Lu, Xiao-Chun;Gong, Hua-Qin;Bai, Shu-Nong
阐明植物器官形成的调控机制是植物发育生物学的重要组成部分，对作物改良具有重要意义。植物器官形成，或器官发生，发生在一组原始细胞分化成一个器官时，通过一系列精心策划的事件，具有给定的形状，结构和功能。过去二十年的研究已经阐明了器官身份和背腹轴决定的分子机制。然而，很少有人知道的分子机制的连续过程。为了在分子水平上研究水稻雄蕊器官形成的有效途径，我们建立了反映水稻雄蕊早期发育的器官特异性基因表达谱。在这项研究中，我们证明了GEP与早期雄蕊发育高度相关，这表明这种分析是有用的解剖雄蕊发育调控。基于分子和形态学相关性，我们发现超过26个基因可能参与雄蕊发育的调控，这些基因在雄蕊发育早期被优先上调。此外，我们发现，差异表达的基因在早期雄蕊发育过程中聚集成两个分支，表明雄蕊发育可能包括两个不同的阶段的图案形成和细胞分化。此外，基因表达水平的器官特异性定量变化可能在调节植物器官形成中起关键作用。
Elucidating the regulatory mechanisms of plant organ formation is an important component of plant developmental biology and will be useful for crop improvement applications. Plant organ formation, or organogenesis, occurs when a group of primordial cells differentiates into an organ, through a well-orchestrated series of events, with a given shape, structure and function. Research over the past two decades has elucidated the molecular mechanisms of organ identity and dorsalventral axis determinations. However, little is known about the molecular mechanisms underlying the successive processes. To develop an effective approach for studying organ formation at the molecular level, we generated organ-specific gene expression profiles (GEPs) reflecting early development in rice stamen. In this study, we demonstrated that the GEPs are highly correlated with early stamen development, suggesting that this analysis is useful for dissecting stamen development regulation. Based on the molecular and morphological correlation, we found that over 26 genes, that were preferentially up-regulated during early stamen development, may participate in stamen development regulation. In addition, we found that differentially expressed genes during early stamen development are clustered into two clades, suggesting that stamen development may comprise of two distinct phases of pattern formation and cellular differentiation. Moreover, the organ-specific quantitative changes in gene expression levels may play a critical role for regulating plant organ formation.