Physical, chemical, and microbiological changes in the ceca of broiler chickens subjected to incremental feed withdrawal
Physical, chemical, and microbiological changes in the ceca of broiler chickens subjected to incremental feed withdrawal
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DOI：
10.1093/ps/79.4.483
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发表时间：
2000-04-01
期刊：
影响因子：
4.4
通讯作者：
Ingram, KD
中科院分区：
文献类型：
--
作者：
Hinton, A;Buhr, RJ;Ingram, KD
进行试验以确定停饲对上市年龄肉鸡盲肠中的重量、pH、天然细菌植物群和鼠伤寒沙门氏菌的持久性的影响。向肉鸡提供加药或未加药饲料，然后在运输板条箱或垫料中停喂0至24 h。停饲后，将肉鸡击昏、流血的、烫伤并挑选。无菌取出每只鸡的一个盲肠并称重。然后将盲肠混合在20 ml蒸馏水中，并测量混合悬浮液的pH。总需氧菌数、肠杆菌科、沙门氏菌属、沙门氏菌属;在适当的细菌学培养基上计数悬浮液中的鼠伤寒沙门氏菌和乳酸菌。结果表明，长达24小时的饲料撤回盲肠重量没有产生显着的变化，盲肠pH值变化高达0.3个单位，在饲料撤回。在试验2和3中，停饲期间肠杆菌科的种群显著增加，并且在试验3中盲肠需氧菌的种群显著增加。在所有试验中，停饲均使乳酸菌数量显著减少，但对S.鼠伤寒发生在饲料停药期间。盲肠重量、pH值、本地细菌数量或S.鼠伤寒杆菌种群之间的肉鸡进行饲料撤回窝或在板条箱。研究结果表明，饲料撤回并不总是有效地疏散盲肠的内容物，并进行饲料撤回的肉鸡盲肠可以保持食源性细菌病原体的来源。
Trials were conducted to determine the effect of feed withdrawal on the weight, pH, native bacterial flora, and the persistence of Salmonella typhimurium in the ceca of market-age broilers. Broilers were provided medicated or unmedicated feed and then were subjected to feed withdrawal for 0 to 24 h in transportation crates or on litter. After feed withdrawal, broilers were stunned, bled, scalded, and picked. One cecum from each bird was aseptically removed and weighed. The cecum was then blended in 20 mt of distilled water, and the pH of the blended suspension was measured. The number of total aerobes, Enterobacteriaceae, S. typhimurium, and lactic acid bacteria in the suspension were enumerated on the appropriate bacteriological media. Results indicated that up to 24 h of feed withdrawal produced no significant change in cecal weight and that cecal pH varied by up to 0.3 units during feed withdrawal. There were significant increases in the population of Enterobacteriaceae during feed withdrawal in Trials 2 and 3, and there was a significant increase in the population of cecal aerobes in Trial 3. Feed withdrawal produced significant decreases in the population of lactic acid bacteria in all trials, but no significant change in the population of S. typhimurium occurred during feed withdrawal. There were no significant differences in cecal weight, pH, native bacteria populations, or S. typhimurium populations between broilers that were subjected to feed withdrawal on litter or in crates. Findings indicate that feed withdrawal does not always effectively evacuate the contents of the ceca and that the ceca of broilers subjected to feed withdrawal can remain a source of foodborne bacterial pathogens.