CLONED HUMAN LYMPHOCYTES-T WITH LYMPHOSTIMULATORY CAPACITY PREFERENTIALLY ACTIVATE SUPPRESSOR CELLS
CLONED HUMAN LYMPHOCYTES-T WITH LYMPHOSTIMULATORY CAPACITY PREFERENTIALLY ACTIVATE SUPPRESSOR CELLS
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DOI：
10.1002/eji.1830140411
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发表时间：
1984-01-01
影响因子：
5.4
通讯作者：
WERNET, P
中科院分区：
文献类型：
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作者：
PAWELEC, G;SCHNEIDER, EM;WERNET, P
来自同种异体混合白细胞培养物（MLC）的一部分克隆T细胞被发现刺激自体以及同种异体外周血单核细胞（PBMC）的快速原发性，但不是继发性淋巴增殖反应。8个主要组织相容性复合体（MHC）II类特异性单克隆抗体（mAb），试剂TU 39（优先抑制SB-而不是DR-或DC-相关的决定因素）最强烈地抑制刺激这些克隆。对DC或DR分子特异性的MAb抑制较弱或根本不抑制。刺激，但不是nonstimulatory，克隆强烈抑制滴定时直接进入MLC。抑制不是MHC限制性的，是抗辐射的（20戈伊），并且不能通过向测试培养物中加入部分纯化的白细胞介素2来消除。转移PBMC共培养的刺激，但不是与nonstimulatory，克隆到第二MLC导致其强烈的抑制，这表明抑制效应人口已诱导的克隆。显然，SB样，而不是DR或DC，存在于某些活化的T细胞表面的决定簇通过在正常淋巴细胞群中快速诱导抑制效应细胞而密切参与细胞免疫应答的调节。
A proportion of cloned T cells derived from allogeneic mixed leukocyte cultures (MLC) was found to stimulate rapid primary, but not secondary, lymphoproliferative responses of autologous as well as allogeneic peripheral blood mononuclear cells (PBMC). Of 8 major histocompatibility complex (MHC) class II-specific monoclonal antibodies (mAb), the reagent TU39 (which preferentially inhibits allostimulation by SB- rather than DR- or DC-associated determinants) most strongly inhibited stimulation by these clones. MAb specific for DC or DR molecules inhibited weakly or not at all. Stimulatory, but not nonstimulatory, clones were strongly suppressive when titrated directly into MLC. Suppression was not MHC restricted, was radioresistant (20 Gy) and was not abrogated by the addition of partially purified interleukin 2 to the test cultures. Transfer of PBMC cocultured with stimulatory, but not with nonstimulatory, clones into a 2nd MLC resulted in its strong suppression, suggesting that a suppressor effector population had been induced by the clones. Apparently, SB-like, rather than DR or DC, determinants present on the surface of certain activated T cells are intimately involved in the regulation of cellular immune responses by rapidly inducing suppressor effector cells in normal lymphocyte populations.